Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Appl Microbiol Biotechnol. 2010 May;86(5):1281-92. doi: 10.1007/s00253-010-2524-4. Epub 2010 Mar 23.
Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiological products. Various other techniques, such as viable staining, in situ hybridization, and those using autofluorescence proteins, are frequently combined with these single-cell isolation techniques depending on the purpose of the study. In this review article, we summarize currently available single-cell isolation techniques and their applications, when used in combination with other techniques, in microbiological and biotechnological studies.
已经开发出了各种单细胞分离技术，包括稀释、显微操作、流式细胞术、微流控技术和分隔化。这些技术可用于培养以前无法培养的微生物，评估和监测细胞生理学和功能，并筛选新型微生物产品。根据研究目的的不同，还经常将各种其他技术，如活细胞染色、原位杂交和使用自发荧光蛋白的技术与这些单细胞分离技术结合使用。在这篇综述文章中，我们总结了目前可用的单细胞分离技术及其应用，以及在微生物学和生物技术研究中与其他技术结合使用时的应用。
