从常规固定的染色体涂片进行特定DNA序列的PCR扩增。 - Suppr

Suppr

超能文献

PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads.

作者信息

Jonveaux P

机构信息

INSERM U.301, IGM, Paris, France.

出版信息

Nucleic Acids Res. 1991 Apr 25;19(8):1946. doi: 10.1093/nar/19.8.1946.

DOI:10.1093/nar/19.8.1946

PMID:2030973

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC328132/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bdfe/328132/83348415f8ec/nar00088-0203-a.jpg

相似文献

PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads.从常规固定的染色体涂片进行特定DNA序列的PCR扩增。

Nucleic Acids Res. 1991 Apr 25;19(8):1946. doi: 10.1093/nar/19.8.1946.

A rapid method for PCR amplification of DNA directly from cells fixed in Carnoy's fixative.一种直接从卡诺氏固定液固定的细胞中进行DNA的PCR扩增的快速方法。

Am J Med Genet. 1995 Jan 2;55(1):116-9. doi: 10.1002/ajmg.1320550130.

Simultaneous evaluation of T- and B-cell clonality, t(11;14) and t(14;18), in a single reaction by a four-color multiplex polymerase chain reaction assay and automated high-resolution fragment analysis: a method for the rapid molecular diagnosis of lymphoproliferative disorders applicable to fresh frozen and formalin-fixed, paraffin-embedded tissues, blood, and bone marrow aspirates.通过四色多重聚合酶链反应分析和自动高分辨率片段分析在单一反应中同时评估T细胞和B细胞克隆性、t(11;14)和t(14;18)：一种适用于新鲜冷冻和福尔马林固定、石蜡包埋组织、血液和骨髓穿刺液的淋巴增殖性疾病快速分子诊断方法。

Am J Pathol. 2001 Dec;159(6):2031-43. doi: 10.1016/S0002-9440(10)63055-6.

PCR amplification of HIV and cellular DNA sequences in formaldehyde-fixed, immunoreactive white blood cells.

Biotechniques. 1993 Jul;15(1):128-33.

An improved strategy and a useful housekeeping gene for RNA analysis from formalin-fixed, paraffin-embedded tissues by PCR.

Biotechniques. 1993 Mar;14(3):448-53.

Sequence analysis of polymerase chain reaction amplified t(14;18) chromosomal breakpoints in formalin fixed, paraffin wax embedded follicular lymphoma.福尔马林固定、石蜡包埋的滤泡性淋巴瘤中聚合酶链反应扩增的t(14;18)染色体断点的序列分析

J Clin Pathol. 1992 Mar;45(3):210-2. doi: 10.1136/jcp.45.3.210.

PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.从单个多线染色体带显微切割的DNA的PCR扩增：与传统微克隆的比较。

Nucleic Acids Res. 1989 Nov 25;17(22):9027-37. doi: 10.1093/nar/17.22.9027.

The effect of fixation type on DNA extracted from paraffin-embedded tissue for PCR studies in dermatopathology.固定类型对从石蜡包埋组织中提取用于皮肤病理学PCR研究的DNA的影响。

Dermatology. 1997;195(2):105-7. doi: 10.1159/000245709.

Amplification of herpes simplex virus type 1 DNA in human geniculate ganglia from formalin-fixed, nonembedded temporal bones.在福尔马林固定、未包埋的颞骨的人膝状神经节中单纯疱疹病毒1型DNA的扩增

Otolaryngol Head Neck Surg. 2000 Oct;123(4):508-11. doi: 10.1067/mhn.2000.107886.

Comparison of formalin, ethanol, and Histochoice fixation on the PCR amplification from paraffin-embedded breast cancer tissue.福尔马林、乙醇和Histochoice固定剂对石蜡包埋乳腺癌组织PCR扩增的比较。

Eur J Clin Chem Clin Biochem. 1997 Aug;35(8):633-5.

引用本文的文献

CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens.使用从固定细胞遗传学标本和长期冷藏骨髓标本中提取的DNA进行比较基因组杂交（CGH）和单核苷酸多态性（SNP）阵列分析。

Mol Cytogenet. 2012 Feb 2;5:10. doi: 10.1186/1755-8166-5-10.

Trisomy of human chromosome 18: molecular studies on parental origin and cell stage of nondisjunction.人类18号染色体三体：关于不分离的亲本来源和细胞阶段的分子研究

Hum Genet. 1996 Feb;97(2):218-23. doi: 10.1007/BF02265269.

Retrospective study of the parental origin of the extra chromosome in trisomy 18 (Edwards syndrome).18三体综合征（爱德华兹综合征）中额外染色体亲本来源的回顾性研究。

Hum Genet. 1993 Oct;92(4):347-9. doi: 10.1007/BF01247332.

本文引用的文献

Isolation and characterization of DNA from fixed cells and tissues.

Exp Cell Res. 1968 Apr;50(1):47-53. doi: 10.1016/0014-4827(68)90392-3.

High molecular weight DNA from fixed cytogenetic preparations.来自固定细胞遗传学标本的高分子量DNA。

Am J Hum Genet. 1986 Nov;39(5):661-8.

Characterization of the human p53 gene.人类p53基因的特征描述

Mol Cell Biol. 1986 May;6(5):1379-85. doi: 10.1128/mcb.6.5.1379-1385.1986.

Chromosomal deletions in non-Hodgkin's malignant lymphomas.非霍奇金恶性淋巴瘤中的染色体缺失

Cancer Genet Cytogenet. 1990 Oct 15;49(2):265-9. doi: 10.1016/0165-4608(90)90151-y.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验