College of Food and Bioengineering, South China University of Technology, Guangzhou, PR China.
J Sep Sci. 2010 May;33(9):1349-55. doi: 10.1002/jssc.200900776.
Citrus aurantium L. var. amara Engl is a member of genus Citrus (Rutaceae) and has been used in Chinese medicine with the effectiveness of digestant and expectorant. Ultrasonic-assisted extraction process for maximum flavonoids from the flower of Citrus aurantium L. var. amara Engl was investigated by response surface methodology. Through single factor experiment, ranges of the main variables (including ethanol concentration, solid/liquid ratio, extraction time and temperature) affecting the extraction yield of flavonoids were confirmed. Box-Behnken central composite design consisting of 24 experimental runs and 5 replicates at zero point was then applied and a regress model was obtained to predict the optimal extraction yield. The ANOVA indicated that the regression equation fits very well with the actual situation, reflecting the relationship between the extraction yield of flavonoids and extraction conditions. The optimal conditions were as follows: extraction temperature 72.11 degrees C, time 51.89 min, ethanol concentration 51.19% and liquid/solid ratio of 40:10. Under the optimal conditions, the maximum response value of yield (1.88%) was consistent with the experimental value (1.87%), indicating the feasibility and validation of response surface methodology in optimizing the extraction of flavonoids from the flower of Citrus aurantium L. var. amara Engl.
酸橙 L. var. amara Engl 是芸香科柑橘属的一员，在中国医学中被用作助消化和祛痰药。采用响应面法研究了从酸橙 L. var. amara Engl 的花中提取黄酮类化合物的超声辅助提取工艺。通过单因素实验，确定了影响黄酮类化合物提取率的主要变量（包括乙醇浓度、固液比、提取时间和温度）的范围。然后应用包含 24 个实验运行和 5 个零点重复的 Box-Behnken 中心复合设计，并获得回归模型来预测最佳提取率。方差分析表明，回归方程与实际情况非常吻合，反映了黄酮类化合物的提取率与提取条件之间的关系。最佳条件为：提取温度 72.11℃、时间 51.89min、乙醇浓度 51.19%、液固比 40:10。在最佳条件下，产率的最大响应值（1.88%）与实验值（1.87%）一致，表明响应面法在优化酸橙 L. var. amara Engl 花中黄酮类化合物提取方面的可行性和有效性。
