Birkin J A, Lowe J S, Fry J R, Millard L G
Department of Physiology and Pharmacology, Queen's Medical Centre, Nottingham, UK.
Clin Exp Dermatol. 1991 Mar;16(2):90-7. doi: 10.1111/j.1365-2230.1991.tb00315.x.
Protein and N-linked glycoprotein biosynthesis was studied in histologically verified normal epidermis, actinic keratoses, keratoacanthoma, intra-epidermal carcinoma and squamous-cell carcinoma using polyacrylamide gel electrophoresis (PAGE). The PAGE profiles of 3[H]-leucine-labelled proteins and 3[H]-mannose-labelled glycoprotein from all disease states studied differed from each other and from normal epidermis. A large 3[H]-mannose-labelled glycoprotein region (band A) with a peak at 97-92 kDa appeared to indicate the presence of a relatively large proportion of basaloid cells in the tissue. An associated peak in the region of 78-74 kDa also appeared in normal epidermis and what appeared to be non-invasive lesions. The main region of change in all lesions corresponded to the 66-34-kDa region (bands B and C). The absence of a group of glycoproteins and proteins in the 62-58-kDa region appeared to be specific for invasive squamous-cell carcinoma. All tumours showed a peak at 38-34 kDa which was not present in normal epidermis. Actinic keratosis had a pattern similar to normal epidermis except that the peaks of band B tended towards the higher-molecular-weight end of the band than those in normal epidermis and peaks at 28-22 kDa were seen. The latter seemed to correspond to the presence of a high proportion of spinous cells in the tissue sample.
使用聚丙烯酰胺凝胶电泳（PAGE）对组织学证实的正常表皮、光化性角化病、角化棘皮瘤、表皮内癌和鳞状细胞癌中的蛋白质和N-连接糖蛋白生物合成进行了研究。在所研究的所有疾病状态下，用³H-亮氨酸标记的蛋白质和³H-甘露糖标记的糖蛋白的PAGE图谱彼此不同，且与正常表皮不同。一个大的³H-甘露糖标记糖蛋白区域（A带），其峰值在97 - 92 kDa，似乎表明组织中存在相对较大比例的基底样细胞。在正常表皮和看似非侵袭性病变中，78 - 74 kDa区域也出现了一个相关峰值。所有病变的主要变化区域对应于66 - 34 kDa区域（B带和C带）。62 - 58 kDa区域中一组糖蛋白和蛋白质的缺失似乎是侵袭性鳞状细胞癌所特有的。所有肿瘤在38 - 34 kDa处都有一个峰值，而正常表皮中不存在该峰值。光化性角化病的模式与正常表皮相似，只是B带的峰值比正常表皮中的峰值更倾向于该带的高分子量端，并且在28 - 22 kDa处可见峰值。后者似乎对应于组织样本中存在高比例的棘细胞。
