Departamento de Química- Física Aplicada, Area de Tecnología de los Alimentos, Facultad Ciencias, Universidad Autónoma de Madrid, Madrid, Spain.
Food Chem Toxicol. 2010 Jun;48(6):1568-75. doi: 10.1016/j.fct.2010.03.026. Epub 2010 Mar 21.
Two fractions (S1 and S2) of an oregano (Origanum vulgare) extract obtained by supercritical fluid extraction have been used to test anti-inflammatory effects on activated human THP-1 cells. The main compounds present in the supercritical extract fractions of oregano were trans-sabinene hydrate, thymol and carvacrol. Fractions toxicity was assessed using the mitochondrial-respiration-dependent 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction method for several concentrations during 24 and 48 h of incubation. Concentrations higher than 30 microg/mL of both supercritical S1 and S2 oregano fractions caused a reduction in cell viability in a dose-dependent manner. Oxidized-LDLs (oxLDLs) activated THP-1 macrophages were used as cellular model of atherogenesis and the release/secretion of cytokines (TNT-alpha, IL-1beta, IL-6 and IL-10) and their respective mRNA expressions were quantified both in presence or absence of supercritical oregano extracts. The results showed a decrease in pro-inflammatory TNF-alpha, IL-1beta and IL-6 cytokines synthesis, as well as an increase in the production of anti-inflammatory cytokine IL-10. These results may suggest an anti-inflammatory effect of oregano extracts and their compounds in a cellular model of atherosclerosis.
两种 fractions（S1 和 S2）从超临界流体萃取获得的牛至（牛至）提取物已被用于测试抗炎作用对激活的人 THP-1 细胞。超临界萃取 fractions 中的主要化合物存在牛至 trans-sabinene 水合物、百里酚和香芹酚。fractions 毒性进行了评估使用线粒体呼吸依赖 3-（4,5-二甲基噻唑-2-yl）-2,5-二苯基四氮唑（MTT）减少方法为几个浓度在 24 和 48 h 孵育。浓度高于 30 microg/mL 的超临界 S1 和 S2 牛至 fractions 引起细胞活力降低呈剂量依赖性。氧化低密度脂蛋白（oxLDLs）激活的 THP-1 巨噬细胞作为动脉粥样硬化的细胞模型和释放/分泌细胞因子（TNF-alpha、IL-1beta、IL-6 和 IL-10）和它们各自的 mRNA 表达都在存在或不存在超临界牛至提取物的情况下进行了定量。结果表明，促炎细胞因子 TNF-alpha、IL-1beta 和 IL-6 的合成减少，抗炎细胞因子 IL-10 的产生增加。这些结果可能表明牛至提取物及其化合物在动脉粥样硬化的细胞模型中具有抗炎作用。
