Avram Ștefana, Bora Larisa, Vlaia Lavinia Lia, Muț Ana Maria, Olteanu Gheorghe-Emilian, Olariu Ioana, Magyari-Pavel Ioana Zinuca, Minda Daliana, Diaconeasa Zorița, Sfirloaga Paula, Adnan Mohd, Dehelean Cristina Adriana, Danciu Corina
Department of Pharmacognosy, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.
Research Center for Pharmaco-Toxicological Evaluation, Victor Babes University of Medicine and Pharmacy, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania.
Pharmaceuticals (Basel). 2023 Jun 29;16(7):940. doi: 10.3390/ph16070940.
var. essential oil (OEO) is known as a natural product with multiple beneficial effects with application in dermatology. Oregano essential oil represents a potential natural therapeutic alternative for fibroepithelial polyps (FPs), commonly known as skin tags. Innovative formulations have been developed to improve the bioavailability and stability of essential oils. In this study, we aimed to evaluate the morphology of a polymeric-micelles-based hydrogel (OEO-PbH), the release and permeation profile of oregano essential oil, as well as to assess in vivo the potential effects on the degree of biocompatibility and the impact on angiogenesis in ovo, using a chick chorioallantoic membrane (CAM). Scanning electron microscopy (SEM) analysis indicated a regular aspect after the encapsulation process, while in vitro release studies showed a sustained release of the essential oil. None of the tested samples induced any irritation on the CAM and the limitation of the angiogenic process was noted. OEO-PbH, with a sustained release of OEO, potentially enhances the anti-angiogenic effect while being well tolerated and non-irritative by the vascularized CAM, especially on the blood vessels (BVs) in the presence of leptin treatment. This is the first evidence of in vivo antiangiogenic effects of a polymeric-micelle-loaded oregano essential oil, with further mechanistic insights for OEO-PbH formulation, involving leptin as a possible target. The findings suggest that the OEO-containing polymeric micelle hydrogel represents a potential future approach in the pathology of cutaneous FP and other angiogenesis-related conditions.
牛至精油（OEO）是一种天然产物，具有多种有益作用，可应用于皮肤病学。牛至精油是纤维上皮息肉（FPs，俗称皮赘）的一种潜在天然治疗替代品。人们已经开发出创新配方来提高精油的生物利用度和稳定性。在本研究中，我们旨在评估基于聚合物胶束的水凝胶（OEO-PbH）的形态、牛至精油的释放和渗透情况，以及使用鸡胚绒毛尿囊膜（CAM）在体内评估其对生物相容性程度的潜在影响和对血管生成的影响。扫描电子显微镜（SEM）分析表明包封过程后形态规则，而体外释放研究显示精油持续释放。所有测试样品均未对CAM引起任何刺激，并且观察到血管生成过程受到限制。OEO-PbH能持续释放OEO，在血管化的CAM存在时，尤其是在有瘦素处理的情况下，对血管（BVs）具有良好的耐受性和无刺激性，同时可能增强抗血管生成作用。这是负载聚合物胶束的牛至精油体内抗血管生成作用的首个证据，为OEO-PbH制剂提供了进一步的机制见解，涉及瘦素作为可能的靶点。研究结果表明，含OEO的聚合物胶束水凝胶是皮肤FP病理学及其他血管生成相关病症未来潜在的治疗方法。
