Glenn J A, Jordan F L, Thomas W E
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
Brain Res Bull. 1989 Jun;22(6):1049-52. doi: 10.1016/0361-9230(89)90018-x.
The investigation of brain microglia in primary cultures of dissociated cerebral cortical cells has been continued here utilizing a histochemical procedure for thiamine pyrophosphatase, which selectively stains microglia in whole tissue. This procedure also selectively stained a subpopulation of cells in the cortical cultures. The stained cells were small and exhibited quite variable morphology; from these features, a corresponding cell population could be identified in viable cultures. The stained cells, which were distinct from previously identified macrophage forms, were also distinguished from similar appearing cell types--small neurons and oligodendrocytes. Based on their staining properties, morphology, and distinction from other cell types, the identified cells are proposed as equivalents of ramified microglia.
利用针对硫胺素焦磷酸酶的组织化学方法，我们在此继续对解离的大脑皮质细胞原代培养物中的脑小胶质细胞进行研究，该方法可选择性地对全组织中的小胶质细胞进行染色。此方法还选择性地对皮质培养物中的一个细胞亚群进行了染色。染色的细胞体积小，形态变化很大；根据这些特征，可以在活细胞培养物中识别出相应的细胞群体。这些染色细胞与先前鉴定的巨噬细胞形式不同，也与外观相似的细胞类型——小神经元和少突胶质细胞区分开来。基于它们的染色特性、形态以及与其他细胞类型的区别，所鉴定出的细胞被认为等同于分支状小胶质细胞。
