Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145, India.
Mol Biol Rep. 2010 Dec;37(8):4075-82. doi: 10.1007/s11033-010-0067-5. Epub 2010 Mar 24.
Finger millet (Eleusine coracana L.) is an important crop used for food, forage, and industrial products. Three DNA marker techniques, random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and cytochrome P(450) gene based markers were used for the detection of genetic polymorphism in 83 accessions of finger millet collected from various geographical regions of India and Africa. A total of 18 RAPD, 10 SSR and 10 pairs of cytochrome P(450) gene based markers were generated 56.17, 70.19 and 54.29% polymorphism, respectively. Mean polymorphism information content (PIC) for each of these marker systems (0.280 for RAPD, 0.89 for SSR and 0.327 for cytochrome P(450) gene based markers) suggested that SSR marker were highly effective in determining polymorphism. The phenograms based on the three markers data indicate that genotypes from different geographical regions are clearly distinguishable as separate clusters. Mantel test employed for detection of goodness of fit established cophenetic correlation values above 0.90 for all the three marker systems. The dendrograms and PCA plots derived from the binary data matrices of the three marker systems are highly concordant. High bootstrap values were obtained at major nodes of phenograms through WINBOOT software. Based on the results of present study, SSR and cytochrome P(450) gene based markers appear to be particularly useful for the estimation of genetic diversity. This study reveals the potential of RAPD, SSR and gene based markers for characterizing germplasm of Eleusine coracana and narrow down the vast germplasm into distinct core groups.
手指小米（Eleusine coracana L.）是一种重要的作物，用于食品、饲料和工业产品。本研究采用三种 DNA 标记技术，即随机扩增多态性 DNA（RAPD）、简单序列重复（SSR）和细胞色素 P（450）基因标记，对来自印度和非洲不同地理区域的 83 份手指小米材料进行遗传多态性检测。共产生了 18 条 RAPD、10 条 SSR 和 10 对细胞色素 P（450）基因标记，多态性分别为 56.17%、70.19%和 54.29%。这些标记系统的平均多态性信息含量（PIC）分别为 0.280（RAPD）、0.89（SSR）和 0.327（细胞色素 P（450）基因标记），表明 SSR 标记在确定多态性方面非常有效。基于这三种标记数据的聚类图表明，来自不同地理区域的基因型可清晰地区分为不同的聚类。Mantel 检验用于检测拟合优度，结果表明所有三种标记系统的 Cophenetic 相关值均高于 0.90。从三种标记系统的二值数据矩阵得出的聚类图和 PCA 图高度一致。通过 WINBOOT 软件，在聚类图的主要节点上获得了较高的自举值。基于本研究结果，SSR 和细胞色素 P（450）基因标记似乎特别有助于遗传多样性的估计。本研究表明 RAPD、SSR 和基因标记可用于鉴定 Eleusine coracana 的种质资源，并将庞大的种质资源缩小到不同的核心群体。
