Yamanaka S, Suzuki E, Tanaka M, Takeda Y, Watanabe J A, Watanabe K N
Gene Research Center, Institute of Biological Sciences, University of Tsukuba, 1-1-1 Tennodai, 305-8572, Tsukuba, Japan.
Theor Appl Genet. 2003 Dec;108(1):1-9. doi: 10.1007/s00122-003-1403-0. Epub 2003 Sep 13.
To investigate and develop new genetic tools for assessing genome-wide diversity in higher plant-species, polymorphisms of gene analogues of mammalian cytochrome P450 mono-oxygenases were studied. Data mining on Arabidopsis thaliana indicated that a small number of primer-sets derived from P450 genes could provide universal tools for the assessment of genome-wide genetic diversity in diverse plant species that do not have relevant genetic markers, or for which, there is no prior inheritance knowledge of inheritance traits. Results from PCR amplification of 51 plant species from 28 taxonomic families using P450 gene-primer sets suggested that there were at least several mammalian P450 gene mammalian-analogues in plants. Intra- and inter- specific variations were demonstrated following PCR amplifications of P450 analogue fragments, and this suggested that these would be effective genetic markers for the assessment of genetic diversity in plants. In addition, BLAST search analysis revealed that these amplified fragments possessed homologies to other genes and proteins in different plant varieties. We conclude that the sequence diversity of P450 gene-analogues in different plant species reflects the diversity of functional regions in the plant genome and is therefore an effective tool in functional genomic studies of plants.
为了研究和开发用于评估高等植物物种全基因组多样性的新遗传工具，对哺乳动物细胞色素P450单加氧酶基因类似物的多态性进行了研究。对拟南芥的数据挖掘表明，少数源自P450基因的引物组可为评估没有相关遗传标记或没有遗传性状遗传先验知识的不同植物物种的全基因组遗传多样性提供通用工具。使用P450基因引物组对来自28个分类科的51种植物进行PCR扩增的结果表明，植物中至少存在几种哺乳动物P450基因的类似物。对P450类似物片段进行PCR扩增后显示出种内和种间变异，这表明这些将是评估植物遗传多样性的有效遗传标记。此外，BLAST搜索分析表明，这些扩增片段与不同植物品种中的其他基因和蛋白质具有同源性。我们得出结论，不同植物物种中P450基因类似物的序列多样性反映了植物基因组功能区域的多样性，因此是植物功能基因组学研究中的有效工具。
