Department of Biotechnology, University of Kerala, Kariavattom, Trivandrum, 695581, India.
Department of Botany, University of Kerala, Kariavattom, Trivandrum, 695581, India.
Mol Biol Rep. 2020 Apr;47(4):2929-2939. doi: 10.1007/s11033-020-05391-w. Epub 2020 Mar 31.
Drumstick (Moringa oleifera Lam.) is an important vegetable as well as forage crop of arid and semi-arid zones of the tropics. The leaves and pods of the plant are rich sources of minerals and vitamins. In the present work, genetic diversity study of 23 genotypes of M. oleifera collected from Kerala, Tamil Nadu and Karnataka states of India was carried out using seven cytochrome P450 (CytP450) markers. By using seven pairs of CytP450 gene-based markers, 88.25% of polymorphism was recorded among the 23 sampled genotypes. The Polymorphic Information Content (PI), Marker Index (MI) and Resolving Power obtained for seven primers were estimated 0.23, 2.96 and 9.83, respectively. The Unweighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram based on this marker data indicate that genotypes from different geographical regions are placed in the same clusters. The dendrogram and Principal Coordinates Analysis (PCoA) plots derived from the binary data matrices were highly concordant. The investigation, in brief, proved that CytP450 based marker system is efficient in the elucidation of genetic diversity in M. oleifera accessions.
鼓槌（辣木）是热带干旱和半干旱地区的一种重要蔬菜和饲料作物。该植物的叶子和豆荚富含矿物质和维生素。在本工作中，使用 7 个细胞色素 P450（CytP450）标记物对来自印度喀拉拉邦、泰米尔纳德邦和卡纳塔克邦的 23 个辣木基因型进行了遗传多样性研究。利用 7 对基于 CytP450 基因的标记物，在 23 个抽样基因型中记录到 88.25%的多态性。估计这 7 个引物的多态信息含量（PI）、标记指数（MI）和分辨率分别为 0.23、2.96 和 9.83。基于该标记数据的非加权对组平均法（UPGMA）聚类图表明，来自不同地理区域的基因型被放置在同一聚类中。源自二值数据矩阵的聚类图和主坐标分析（PCoA）图高度一致。总之，该研究证明了 CytP450 标记系统在阐明辣木属植物遗传多样性方面是有效的。
