Carter M W, al-Mahdawi S A, Giles I G, Treharne J D, Ward M E, Clark I N
Department of Microbiology, University of Southampton, Faculty of Medicine, Southampton General Hospital, UK.
J Gen Microbiol. 1991 Mar;137(3):465-75. doi: 10.1099/00221287-137-3-465.
Chlamydia pneumoniae IOL-207 genomic DNA was hybridized with a 1.5 kb labelled DNA probe containing the 3' region of the coding sequence for the major outer membrane protein (MOMP) of C. trachomatis serovar L1. An 8.5 kb Bg/II fragment containing the complete MOMP gene was cloned into lambda EMBL3. Two hybridizing EcoRI fragments were sub-cloned into the lambda ZAP II cloning vector and the resulting plasmids were used as templates for sequencing both strands of the C. pneumoniae MOMP gene. Computer taxonomic studies using the nucleotide and inferred amino acid sequence of the MOMP of C. pneumoniae IOL-207 and all known chlamydial MOMP sequences supported the designation of C. pneumoniae as a new species, but electron microscope studies suggested that the presence of pear-shaped elementary bodies (EBs) may not be a reliable taxonomic criterion.
肺炎衣原体IOL - 207基因组DNA与一个1.5 kb的标记DNA探针杂交，该探针包含沙眼衣原体L1血清型主要外膜蛋白（MOMP）编码序列的3'区域。一个包含完整MOMP基因的8.5 kb Bg/II片段被克隆到λEMBL3中。两个杂交的EcoRI片段被亚克隆到λZAP II克隆载体中，所得质粒用作肺炎衣原体MOMP基因两条链测序的模板。使用肺炎衣原体IOL - 207的MOMP核苷酸和推导的氨基酸序列以及所有已知衣原体MOMP序列进行的计算机分类学研究支持将肺炎衣原体指定为一个新物种，但电子显微镜研究表明梨形原体（EBs）的存在可能不是一个可靠的分类标准。
