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猪副粘病毒LPMV基质蛋白基因的鉴定:与其他副粘病毒的序列比较分析

Identification of the porcine paramyxovirus LPMV matrix protein gene: comparative sequence analysis with other paramyxoviruses.

作者信息

Berg M, Sundqvist A, Moreno-López J, Linné T

机构信息

Department of Veterinary Microbiology (Molecular Virology), Swedish University of Agricultural Sciences, Uppsala.

出版信息

J Gen Virol. 1991 May;72 ( Pt 5):1045-50. doi: 10.1099/0022-1317-72-5-1045.

DOI:10.1099/0022-1317-72-5-1045
PMID:2033388
Abstract

The complete nucleotide sequence of the gene encoding the matrix protein (M) of the porcine paramyxovirus LPMV has been determined. The gene is 1376 nucleotides long including 5' and 3' non-coding sequences with a protein-coding sequence of 1107 nucleotides. The deduced protein, containing 369 amino acids with a calculated Mr of 41,657, is hydrophobic overall with a net positive charge of +17.5. Comparative sequence analysis revealed high amino acid homology to other paramyxovirus M proteins, with the highest degree of identity (46%) with the human mumps virus. This is strong evidence that the porcine paramyxovirus LPMV is a genuine member of the paramyxovirus genus.

摘要

猪副粘病毒LPMV基质蛋白(M)编码基因的完整核苷酸序列已被确定。该基因长1376个核苷酸,包括5'和3'非编码序列,蛋白质编码序列为1107个核苷酸。推导的蛋白质含有369个氨基酸,计算的分子量为41,657,总体上具有疏水性,净正电荷为+17.5。比较序列分析显示与其他副粘病毒M蛋白具有高度的氨基酸同源性,与人腮腺炎病毒的同源性最高(46%)。这有力地证明了猪副粘病毒LPMV是副粘病毒属的一个真正成员。

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