Gonzalez H A, Knudson D L
J Gen Virol. 1987 Mar;68 ( Pt 3):661-72. doi: 10.1099/0022-1317-68-3-661.
Eight viruses of the Corriparta serogroup (Reoviridae: Orbivirus) that were known to be heterogeneous on the basis of serology and polyacrylamide gel electrophoresis were examined by reciprocal RNA-RNA blot hybridization of genomic RNA. Conserved and variant genes were identified by the degree of hybridization between cognate genes of different isolates. The eight viruses were divided into three subsets on the basis of the number of shared genes. Four of the viruses, isolated in Australia, formed one subset of related isolates and shared five conserved genes. Another isolate, Acado, was variant in all 10 genes and was considered to be a second subset. The remaining three isolates formed a third subset and shared four conserved genes. Genes 1, 3 and 10 were the most variable among the Corriparta serogroup isolates. Subsets of isolates within a serogroup which are highly related in the majority of the 10 genes and less related to serogroup viruses in another subset have not been reported previously. The phylogenetic relationship of Corriparta serogroup members suggested by the blot hybridization data is not apparent in the current taxonomic classification of these viruses which is based primarily upon serological data. The hybridization data on the Corriparta serogroup viruses are discussed and contrasted with other Orbivirus serogroups which have been examined similarly.
基于血清学和聚丙烯酰胺凝胶电泳已知具有异质性的8种科里帕塔血清群病毒（呼肠孤病毒科：环状病毒属），通过基因组RNA的相互RNA-RNA印迹杂交进行了检测。通过不同分离株同源基因之间的杂交程度鉴定保守基因和变异基因。根据共享基因的数量，将这8种病毒分为三个亚组。在澳大利亚分离的4种病毒形成了一个相关分离株亚组，并共享5个保守基因。另一个分离株阿卡多在所有10个基因中均有变异，被认为是第二个亚组。其余3个分离株形成第三个亚组，并共享4个保守基因。基因1、3和10在科里帕塔血清群分离株中变异最大。血清群内分离株亚组在10个基因中的大多数高度相关，而与另一个亚组中的血清群病毒相关性较低，此前尚未见报道。印迹杂交数据表明的科里帕塔血清群成员的系统发育关系，在目前主要基于血清学数据的这些病毒分类中并不明显。讨论了科里帕塔血清群病毒的杂交数据，并与其他经过类似检测的环状病毒血清群进行了对比。
