Takeya M, Tsuchiya T, Shimokawa Y, Takahashi K
Second Department of Pathology, Kumamoto University Medical School, Japan.
J Pathol. 1991 Apr;163(4):315-21. doi: 10.1002/path.1711630408.
A new monoclonal antibody, PM-2K, was raised against 24-h cultured human peritoneal macrophages. Immunohistochemically, PM-2K recognized most tissue macrophages in lymphoreticular organs such as the thymus, spleen, lymph node, and tonsil. Kupffer cells of the liver, alveolar macrophages, and macrophages in the interstitial tissue of the kidney, pancreas, and many other organs were also positively labelled. On the other hand, PM-2K failed to recognize blood monocytes, freshly isolated peritoneal macrophages, microglial cells, osteoclasts, and dendritic cells such as Langerhans cells, interdigitating cells, and follicular dendritic cells. In various pathological conditions, PM-2K labelled a wide variety of proliferating macrophages. Reaction products of PM-2K were observed by immunoelectron microscopy on the cytoplasmic membrane of cultured peritoneal macrophages. The molecular weight of the antigen recognized by PM-2K was determined to be 150 kD by Western blotting. As no cells other than macrophages were reactive with PM-2K, this antibody is considered to be very useful not only in the investigation of macrophage differentiation and maturation, but also in the diagnosis of various proliferative disorders of macrophages.
一种新的单克隆抗体PM - 2K是针对培养24小时的人腹膜巨噬细胞产生的。免疫组织化学研究显示，PM - 2K可识别胸腺、脾脏、淋巴结和扁桃体等淋巴网状器官中的大多数组织巨噬细胞。肝脏的库普弗细胞、肺泡巨噬细胞以及肾脏、胰腺和许多其他器官间质组织中的巨噬细胞也呈阳性标记。另一方面，PM - 2K不能识别血液单核细胞、新鲜分离的腹膜巨噬细胞、小胶质细胞、破骨细胞以及树突状细胞，如朗格汉斯细胞、交错突细胞和滤泡树突状细胞。在各种病理情况下，PM - 2K标记了多种增殖的巨噬细胞。通过免疫电子显微镜在培养的腹膜巨噬细胞的细胞质膜上观察到了PM - 2K的反应产物。通过蛋白质印迹法确定PM - 2K识别的抗原分子量为150 kD。由于除巨噬细胞外没有其他细胞与PM - 2K发生反应，因此该抗体不仅在巨噬细胞分化和成熟的研究中，而且在各种巨噬细胞增殖性疾病的诊断中都被认为非常有用。
