Department of Element Analytics, Helmholtz-Zentrum Berlin für Materialien und Energie, D 14109 Berlin, Germany.
Cancer Genomics Proteomics. 2010 Mar-Apr;7(2):81-6.
To study the metalloproteome of DU-145 prostate carcinoma cells in comparison to prostate from control and selenium-deficient rats.
Total proteome of the samples was compared by two-dimensional electrophoresis (2-DE) and metalloproteome was analysed by size-exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP/MS). Immunotests were used to quantify protein expression of superoxide dismutase, thioredoxin reductase and metallothionein.
There was no general relation between protein expression and metal load. SEC-ICP/MS spectra for many metals varied significantly in terms of peak number and intensity between individuals of the same sample group. However, nickel and zinc peaks were consistently suppressed in DU-145 cells under selenium deficiency. Concurrent redistribution of cobalt binding to a low molecular weight fraction (presumably cobalamin) was observed.
Metal load of proteins in comparison to their expression might point to yet unknown mechanisms of oncogenesis and may lead to new biomarkers of cancer.
与对照和硒缺乏大鼠的前列腺相比，研究 DU-145 前列腺癌细胞的金属蛋白质组。
通过二维电泳（2-DE）比较样品的总蛋白质组，通过尺寸排阻色谱法与电感耦合等离子体质谱（SEC-ICP/MS）分析金属蛋白质组。免疫试验用于定量超氧化物歧化酶、硫氧还蛋白还原酶和金属硫蛋白的蛋白表达。
蛋白质表达与金属负荷之间没有一般关系。在同一样品组的个体之间，SEC-ICP/MS 光谱中许多金属的峰数和强度差异很大。然而，在硒缺乏的情况下，DU-145 细胞中的镍和锌峰一直受到抑制。同时观察到钴与低分子量部分（推测为钴胺素）的重新分布。
与表达相比，蛋白质的金属负荷可能指向尚未知的致癌机制，并可能导致癌症的新生物标志物。
