Faculty of Sciences and Mathematics, Department of Chemistry, University of Nis, Visegradska 33, P.O.Box 224, 18000 Nis, Serbia.
Molecules. 2010 Mar 22;15(3):2016-27. doi: 10.3390/molecules15032016.
The biologically active compounds in wine, especially phenolics, are responsible for reduced risk of developing chronic diseases (cardiovascular disease, cancer, diabetes, etc.), due to their antioxidant activities. We determined the contents of total phenolics (TP) and total flavonoids (TF) in selected Serbian white wines by colorimetric methods. Total antioxidant activity (TAA) of the white wines was analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Medas beli had the highest content of TP, TF and TAA. The radical scavenging capacity (RSC) and total antioxidant activity (TAA) of white wines were 15.30% and 1.055 mM Trolox equivalent, respectively. Total phenolic (TP) and total flavonoid (TF) contents in white wines ranged from 238.3 to 420.6 mg gallic acid equivalent per L of wines and 42.64 to 81.32 mg catechin equivalent per L of wines, respectively. A high and significant correlation between antioxidant activity and total phenolic content was determined in wines (R2 = 0.968, p < 0.01). For the individual polyphenols determination we used a high performance liquid chromatography (HPLC)-diode array detection (DAD) technique. The majority of white wine polyphenols was represent by four hydroxycinnamic acids (HCAs).
葡萄酒中的生物活性化合物,特别是酚类化合物,由于其抗氧化活性,可降低患慢性疾病(心血管疾病、癌症、糖尿病等)的风险。我们通过比色法测定了选定的塞尔维亚白葡萄酒中总酚(TP)和总类黄酮(TF)的含量。使用 2,2-二苯基-1-苦基肼(DPPH)自由基清除能力测定法分析了白葡萄酒的总抗氧化活性(TAA)。Medas beli 具有最高的 TP、TF 和 TAA 含量。白葡萄酒的自由基清除能力(RSC)和总抗氧化活性(TAA)分别为 15.30%和 1.055 mM Trolox 当量。白葡萄酒中的总酚(TP)和总类黄酮(TF)含量范围分别为 238.3 至 420.6 mg 没食子酸当量/升和 42.64 至 81.32 mg 儿茶素当量/升。在葡萄酒中测定了抗氧化活性与总酚含量之间存在高度显著的相关性(R2 = 0.968,p < 0.01)。对于单个多酚的测定,我们使用了高效液相色谱(HPLC)-二极管阵列检测(DAD)技术。白葡萄酒中的多酚主要由四种羟基肉桂酸(HCAs)组成。