College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China.
Molecules. 2011 Jul 19;16(7):5998-6009. doi: 10.3390/molecules16075998.
The antioxidant activities of water extract (WE), ethanol extract (EE), residue water extract (RWE) and petroleum ether (PF), ethyl acetate (EF), n-BuOH (BF) and water (WF) fractions of the ethanol extract from Eupatorium Lindley DC were investigated for the first time. Total phenolics content, DPPH radical scavenging activities, superoxide radical scavenging activities, total reduction capability, and ferrous ions chelating activities were determined for all the extracts and fractions. The results showed that all the extracts and fractions exhibited antioxidant activities with different magnitudes of potency. Among all the samples, WE and RWE exhibited the best antioxidant capacities, the BF also exhibited high antioxidant abilities in all tests except for the metal chelating activity, while the other extracts and fractions were relatively weak antioxidants. The BF had the highest total phenolics contents in all extracts and fractions, and the WE and RWE were found to be rich in tannins. Furthermore, the content of total phenolics showed good correlation with DPPH radical scavenging activity, superoxide anion radical scavenging activity, and the reducing power. Phenolic composition of all the extracts and fractions was identified and quantified by HPLC. The results indicate that the extracts of E. Lindley DC might be a useful potential source of natural antioxidant ingredients.
首次研究了从紫菀 Lindley DC 的乙醇提取物中分离得到的水提取物 (WE)、乙醇提取物 (EE)、残渣水提取物 (RWE) 以及石油醚 (PF)、乙酸乙酯 (EF)、正丁醇 (BF) 和水 (WF) 各部分的抗氧化活性。测定了所有提取物和馏分的总酚含量、DPPH 自由基清除活性、超氧阴离子自由基清除活性、总还原能力和亚铁离子螯合活性。结果表明，所有提取物和馏分均表现出不同程度的抗氧化活性。在所有样品中，WE 和 RWE 表现出最佳的抗氧化能力，BF 在所有测试中除金属螯合活性外均表现出较高的抗氧化能力，而其他提取物和馏分则相对较弱。BF 在所有提取物和馏分中总酚含量最高，WE 和 RWE 被发现富含单宁。此外，总酚含量与 DPPH 自由基清除活性、超氧阴离子自由基清除活性和还原能力呈良好相关性。通过 HPLC 鉴定和定量了所有提取物和馏分的酚类成分。结果表明，紫菀 Lindley DC 的提取物可能是天然抗氧化成分的有用潜在来源。
