Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University, 54124 Thessaloniki, Greece.
Anal Bioanal Chem. 2010 Jul;397(6):2191-7. doi: 10.1007/s00216-010-3639-2. Epub 2010 Mar 25.
A 13-min LC-MS method was developed for the determination of daptomycin, a new potent antibiotic, in peritoneal fluid, blood plasma, and urine of patients receiving renal replacement therapy. Chromatography was performed on a C(18) column and detection was performed by a single-quadrupole mass spectrometer coupled to LC via an electrospray interface (ESI). The column effluent was also monitored at 370 nm using a photodiode-array detector. The developed method provided a linear dynamic range for concentrations from 0.5 microg mL(-1) to 100 microg mL(-1). Method precision and accuracy were found to be satisfactory for clinical application, thus the method was successfully used for the analysis of daptomycin in pharmacokinetic studies. The drug was preventively administered against Gram-positive infections to 19 clinical patients undergoing peritoneal dialysis. Peritoneal fluid, blood plasma, and urine samples were collected at 13 time points over a period of 48 h. Clinical samples were analysed following simple sample-preparation procedures and daptomycin was unambiguously detected and quantified.
建立了一种测定接受肾脏替代治疗患者的腹腔液、血浆和尿液中达托霉素（一种新型强效抗生素）的 13 分钟 LC-MS 方法。色谱在 C(18)柱上进行，通过电喷雾接口（ESI）将 LC 与单四极杆质谱仪进行检测。柱流出物也通过光电二极管阵列检测器在 370nm 处进行监测。所开发的方法提供了 0.5μg/mL 至 100μg/mL 浓度范围内的线性动态范围。该方法的精密度和准确度均满足临床应用的要求，因此该方法成功地用于药代动力学研究中达托霉素的分析。该药物预防性用于革兰氏阳性感染的 19 名接受腹膜透析的临床患者。在 48 小时的时间内采集了 13 个时间点的腹腔液、血浆和尿液样本。临床样本经过简单的样品前处理程序进行分析，可明确检测和定量达托霉素。
