Chen Mei-Jung, Peng Mei-Lin, Chen Li-Wen, Fan Hsiao-Lin, Liou Ying-Ming, Liao Jiuan-Miaw
Department of Medical Engineering, Ming Chuan University, Taoyuan, Taiwan, Republic of China.
Chin J Physiol. 2009 Dec 31;52(6):426-31. doi: 10.4077/cjp.2009.amh069.
The pressor effects on blood pressure (BP) elicited by electro-acupuncture (Ea) stimulations and vesico-vascular reflex (VVR) were investigated in anesthetized rats. Twenty adult female Sprague-Dawley rats were used throughout this study. Two acupoints, the Hoku (Li-4) and the Tsusanli (St-36), were tested by a low frequency Ea (LFEa, 2 Hz) and a high frequency Ea (HFEa, 20 Hz) with intensities 20 times that of the motor threshold. Ea at Tsusanli elicited no pressor effects (98.15 +/- 4.10% and 101.43 +/- 3.96% of pre-stimulation control in LFEa and HFEa, respectively) whereas pressor effects could be induced by Ea stimulations at Hoku (126.3 +/- 3.3% and 136.3 +/- 3.8% of pre-stimulation control in LFEa and HFEa, respectively, P < 0.01, n=10). In addition, the patterns of pressor effects elicited by LFEa and HFEa at Hoku were different, i.e., LFEa at Hoku elicited a tonic pressor effect, while HFEa elicited a phasic one. The VVR induced by bladder isovolumic saline distension also elicited a pressor effect on BP (119.2 +/- 2.2%, P < 0.01, n=10) in the same preparations during bladder contractions. The VVR did not modify the Ea-induced pressor responses, and vice versa, when both of them were superimposed. All the results suggested that the pressor effects elicited by the VVR and the Ea stimulation were additive responses. In addition, for future clinical application, our findings may imply that patients should be carefully monitored for signs and symptoms of autonomic dysfunction during clinical acupuncture treatments.
在麻醉大鼠中研究了电针（Ea）刺激和膀胱血管反射（VVR）对血压（BP）的升压作用。本研究使用了20只成年雌性Sprague-Dawley大鼠。通过低频Ea（LFEa，2Hz）和高频Ea（HFEa，20Hz）测试了两个穴位，即合谷（LI-4）和足三里（ST-36），强度为运动阈值的20倍。足三里的Ea未引起升压作用（LFEa和HFEa分别为刺激前对照的98.15±4.10%和101.43±3.96%），而合谷的Ea刺激可诱导升压作用（LFEa和HFEa分别为刺激前对照的126.3±3.3%和136.3±3.8%，P<0.01，n=10）。此外，合谷的LFEa和HFEa引起的升压作用模式不同，即合谷的LFEa引起持续性升压作用，而HFEa引起阶段性升压作用。在相同的实验准备中，膀胱等容生理盐水扩张诱导的VVR在膀胱收缩期间也对BP产生了升压作用（119.2±2.2%，P<0.01，n=10）。当VVR和Ea诱导的升压反应叠加时，VVR不改变Ea诱导的升压反应，反之亦然。所有结果表明，VVR和Ea刺激引起的升压作用是相加反应。此外，对于未来的临床应用，我们的发现可能意味着在临床针灸治疗期间应仔细监测患者自主神经功能障碍的体征和症状。
