Department of Chemistry, Pusan National University, Pusan 609-735, South Korea.
Anal Chem. 2009 Aug 15;81(16):6604-11. doi: 10.1021/ac900285v.
Au nanoparticles-doped conducting polymer nanorods electrodes (AuNPs/CPNEs) were prepared by coating Au nanorods (AuNRs) with a conducting polymer layer. The AuNRs were prepared through an electroless deposition method using the polycarbonate membrane (pore diameter, 50 nm, pore density, 6 x 10(8) pores/cm(2)) as a template. The AuNPs/CPNEs combining catalytic activity of ferrocene to ascorbic acid were used for the fabrication of an ultrasensitive aptamer sensor for thrombin detection. The AuNPs/3D-CPNEs were characterized employing cyclic voltammetry (CV), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and atomic force microscopy (AFM). Sandwiched immunoassay for alpha-human thrombin with NH(2)-functionalized-thrombin binding aptamer (Apt) immobilized on AuNPs/3D-CPNEs was studied through the electrocatalytic oxidation of ascorbic acid by the ferrocene moiety that was bound with an antithrombin antibody and attached with the Apt/3D-CPNEs probe through target binding. Various experimental parameters affecting thrombin detection were optimized, and the performance of the thrombin aptamer sensor was examined. The Apt/AuNPs/3D-CPNEs based thrombin sensor exhibited a wide dynamic range of 5-2000 ng L(-1) and a low detection limit of 5 ng L(-1) (0.14 pM). The selectivity and the stability of the proposed thrombin aptamer sensor were excellent, and it was tested in a real human serum sample for the detection of spiked concentrations of thrombin.
金纳米粒子掺杂的导电聚合物纳米棒电极（AuNPs/CPNEs）是通过在金纳米棒（AuNRs）上涂覆导电聚合物层制备的。AuNRs 通过无电沉积方法制备，使用聚碳酸酯膜（孔径为 50nm，孔密度为 6x10(8)孔/cm(2))作为模板。将具有抗坏血酸氧化酶活性的二茂铁与 AuNPs/CPNEs 结合，用于构建超灵敏适体传感器以检测凝血酶。采用循环伏安法（CV）、X 射线光电子能谱（XPS）、扫描电子显微镜（SEM）和原子力显微镜（AFM）对 AuNPs/3D-CPNEs 进行了表征。通过与抗凝血酶抗体结合并通过靶标结合附着在 Apt/3D-CPNEs 探针上的二茂铁部分，对固定在 AuNPs/3D-CPNEs 上的 NH(2)-功能化凝血酶结合适体（Apt）的 alpha-人凝血酶进行夹心免疫测定，研究了抗坏血酸的电催化氧化。优化了影响凝血酶检测的各种实验参数，并对凝血酶适体传感器的性能进行了检验。基于 Apt/AuNPs/3D-CPNEs 的凝血酶传感器具有 5-2000ng L(-1) 的宽动态范围和 5ng L(-1)（0.14pM）的低检测限。所提出的凝血酶适体传感器具有出色的选择性和稳定性，已在真实的人血清样本中用于检测加入的凝血酶浓度。
