Department of Chemistry, University of California-Irvine, Irvine, California 92697, USA.
Anal Chem. 2010 Apr 15;82(8):3365-70. doi: 10.1021/ac100362u.
Surface patterns of single-stranded DNA (ssDNA) consisting of nanoscale lines as thin as 40 nm were fabricated on polymer substrates for nanotechnology and bioaffinity sensing applications. Large scale arrays (with areas up to 4 cm(2)) of ssDNA "nanolines" were created on streptavidin-coated polymer (PDMS) surfaces by transferring biotinylated ssDNA from a master pattern of gold nanowires attached to a glass substrate. The gold nano-wires were first formed on the glass substrate by the process of lithographically patterned nanowire electrodeposition (LPNE), and then "inked" with biotinylated ssDNA by hybridization adsorption to a thiol-modified ssDNA monolayer attached to the gold nanowires. The transferred ssDNA nanolines were capable of hybridizing with ssDNA from solution to form double-stranded DNA (dsDNA) patterns; a combination of fluorescence and atomic force microscopy (AFM) measurements were used to characterize the dsDNA nanoline arrays. To demonstrate the utility of these surfaces for biosensing, optical diffraction measurements of the hybridization adsorption of DNA-coated gold nanoparticles onto the ssDNA nanoline arrays were used to detect a specific target sequence of unlabeled ssDNA in solution.
用于纳米技术和生物亲和传感应用的单链 DNA（ssDNA）表面图案由厚度薄至 40nm 的纳米线组成，被制备在聚合物基底上。通过将附着在玻璃基底上的金纳米线的主图案中的生物素化 ssDNA 转移到链霉亲和素涂覆的聚合物（PDMS）表面上，在 ssDNA“纳米线”的大阵列（面积高达 4cm2）上形成了 ssDNA“纳米线”。首先，通过光刻图案纳米线电沉积（LPNE）过程在玻璃基底上形成金纳米线，然后通过杂交吸附到附着在金纳米线上的巯基修饰的 ssDNA 单层上将生物素化的 ssDNA“上墨”。转移的 ssDNA 纳米线能够与溶液中的 ssDNA 杂交形成双链 DNA（dsDNA）图案；荧光和原子力显微镜（AFM）测量的组合用于表征 dsDNA 纳米线阵列。为了展示这些表面在生物传感方面的实用性，使用光学衍射测量法测量了 DNA 涂覆的金纳米颗粒在 ssDNA 纳米线阵列上的杂交吸附，以检测溶液中未标记的 ssDNA 的特定目标序列。
