Fujibayashi Kenichi, Hariadi Rizal, Park Sung Ha, Winfree Erik, Murata Satoshi
Department of Computational Intelligence and Systems Science, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8502, Japan.
Nano Lett. 2008 Jul;8(7):1791-7. doi: 10.1021/nl0722830. Epub 2007 Dec 28.
Bottom-up fabrication of nanoscale structures relies on chemical processes to direct self-assembly. The complexity, precision, and yield achievable by a one-pot reaction are limited by our ability to encode assembly instructions into the molecules themselves. Nucleic acids provide a platform for investigating these issues, as molecular structure and intramolecular interactions can encode growth rules. Here, we use DNA tiles and DNA origami to grow crystals containing a cellular automaton pattern. In a one-pot annealing reaction, 250 DNA strands first assemble into a set of 10 free tile types and a seed structure, then the free tiles grow algorithmically from the seed according to the automaton rules. In our experiments, crystals grew to approximately 300 nm long, containing approximately 300 tiles with an initial assembly error rate of approximately 1.4% per tile. This work provides evidence that programmable molecular self-assembly may be sufficient to create a wide range of complex objects in one-pot reactions.
纳米级结构的自下而上制造依赖于化学过程来引导自组装。一锅法反应能够实现的复杂性、精度和产率受到我们将组装指令编码到分子本身能力的限制。核酸为研究这些问题提供了一个平台，因为分子结构和分子内相互作用可以编码生长规则。在这里，我们使用DNA瓦片和DNA折纸来生长包含细胞自动机图案的晶体。在一锅退火反应中，250条DNA链首先组装成一组10种游离瓦片类型和一个种子结构，然后游离瓦片根据自动机规则从种子开始算法式生长。在我们的实验中，晶体生长到大约300纳米长，包含大约300个瓦片，每个瓦片的初始组装错误率约为1.4%。这项工作提供了证据，表明可编程分子自组装可能足以在一锅法反应中创建各种复杂物体。
