Invertebrate Gene Function Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan.
Insect Mol Biol. 2010 Jun 1;19(3):399-408. doi: 10.1111/j.1365-2583.2010.01001.x. Epub 2010 Mar 19.
Juvenile hormone epoxide hydrolases (JHEHs) degrade juvenile hormones (JHs) and are important for JH titre regulation. Here, we report the cloning and analysis of five jheh-related (jheh-r1-r5) genes in the red flour beetle, Tribolium castaneum, a model species for the coleopteran insects. T. castaneum JHEH-r (TcJHEH-r) proteins show high homology to lepidopteran JHEHs and also to human microsomal epoxide hydrolase. In the phylogenetic tree, Tcjheh-rs were clustered, and interestingly, they were also clustered in the genome. Examination of enzymatic activities using recombinant TcJHEH-r proteins showed that TcJHEH-r3 had strong degradation activity for JH III, whereas TcJHEH-r4 had weak activity. The study has yielded significant information that will facilitate further analysis of JHEHs and epoxide hydrolases.
保幼激素环氧化物水解酶（JHEHs）降解保幼激素（JHs），对 JH 浓度的调节很重要。在这里，我们报道了在模式昆虫鞘翅目赤拟谷盗中五个 jheh 相关（jheh-r1-r5）基因的克隆和分析。赤拟谷盗 JHEH-r（TcJHEH-r）蛋白与鳞翅目 JHEHs 以及人类微粒体环氧化物水解酶具有高度同源性。在系统发育树中，Tcjheh-rs 被聚类，有趣的是，它们也在基因组中聚类。使用重组 TcJHEH-r 蛋白检测酶活性表明，TcJHEH-r3 对 JH III 具有很强的降解活性，而 TcJHEH-r4 活性较弱。这项研究提供了重要的信息，将有助于进一步分析 JHEHs 和环氧化物水解酶。
