Department of Mechanical Engineering, College of Engineering, Chosun University, GwangJu, South Korea.
J Biomater Sci Polym Ed. 2010;21(5):553-62. doi: 10.1163/156856209X429166.
Small intestinal submucosa (SIS) has the potential for use as a natural scaffold material because of the presence of type-I and -III collagen and various cytokines. However, although the presence of growth factors in SIS leads to superior initial cell attachment and proliferation compared to synthetic polymeric scaffolds, the lack of reliable and reproducible shape controllability is a drawback. To overcome this problem, SIS was electrospun with a biodegradable and biocompatible polymer, polycaprolactone (PCL). PCL/SIS fibrous webs were fabricated with an electrospinning process using an auxiliary conical electrode to create stable micro/nanofibrous scaffolds. The hydrophilicity, mechanical properties and cellular behavior of the PCL/SIS fibrous scaffold were analyzed. In addition, aligned PCL/SIS fibrous webs were fabricated using various collector rotation speeds. As the alignment of micro/nanofibers increased, the hydrophilicity, orthotropic mechanical properties, and cellular behavior of PC-12 cells improved. These results show the potential for using PCL/SIS fibrous scaffolds as a good natural biomaterial.
小肠黏膜下层（SIS）由于存在 I 型和 III 型胶原和各种细胞因子，因此具有用作天然支架材料的潜力。然而，尽管 SIS 中存在生长因子可导致与合成聚合物支架相比初始细胞附着和增殖更好，但缺乏可靠和可重复的形状可控性是一个缺点。为了克服这个问题，用可生物降解和生物相容的聚合物聚己内酯（PCL）对 SIS 进行了静电纺丝。使用辅助锥形电极通过静电纺丝工艺制造了 PCL/SIS 纤维网，以制造稳定的微/纳米纤维支架。分析了 PCL/SIS 纤维支架的亲水性、机械性能和细胞行为。此外，还使用各种收集器旋转速度制造了定向 PCL/SIS 纤维网。随着微/纳米纤维的取向度增加，PC-12 细胞的亲水性、各向异性机械性能和细胞行为得到改善。这些结果表明，PCL/SIS 纤维支架作为一种良好的天然生物材料具有潜力。
