Department of Biomedical Engineering, Center on Biomaterials, Drug Delivery, Bionanotechnology and Molecular Recognition, 1 University Station, C-0400, The University of Texas at Austin, Austin, TX 78712-0231, USA.
J Biomater Sci Polym Ed. 2010;21(5):623-34. doi: 10.1163/156856209X434647.
Conductive polymers can be utilized as unique functional elements in future intelligent biomaterials required for tissue engineering, cell stimulation and drug delivery. Research in these areas could be limited by established methods of conductive polymer synthesis which do not provide an easy route towards large scale processing of these materials. Typically, conductive polymers are highly insoluble in biocompatible solvents, and can be mechanically brittle, making their integration with biomaterials challenging. As an alternative, the use of a water-soluble conductive polymer for integration with biomaterials, a polyaniline template synthesized with poly(2-acrylamido-2-methylpropanesulfonic acid) (PANI-PAAMPSA), is proposed. To address key fundamental questions about the biocompatibility of this conductive polymer, cell cytotoxicity and proliferation assays have been performed on NIH 3T3 fibroblasts cultured on films of PANI-PAAMPSA. It was determined that the cell cultures maintained growth habits similar to those cultured on the control surfaces. Additionally, conductivity of the PANI-PAAMPSA films subsequent to exposure to the cell culture was demonstrated, indicating the materials retain functionality after cell growth. These results indicate that this type of template synthesized PANI could be successfully implemented as a functional, conductive biomaterial.
导电聚合物可用作组织工程、细胞刺激和药物输送等未来智能生物材料所需的独特功能元素。这些领域的研究可能会受到导电聚合物合成既定方法的限制，这些方法无法为这些材料的大规模处理提供简单的途径。通常，导电聚合物在生物相容性溶剂中高度不溶，并且可能具有机械脆性，因此与生物材料的集成具有挑战性。作为替代方案，提出了使用水溶性导电聚合物与生物材料集成，即用聚（2-丙烯酰胺基-2-甲基丙磺酸）（PANI-PAAMPSA）合成的聚苯胺模板。为了解决有关这种导电聚合物生物相容性的关键基本问题，在 PANI-PAAMPSA 薄膜上培养 NIH 3T3 成纤维细胞，进行了细胞毒性和增殖测定。结果表明，细胞培养保持与对照表面上培养的细胞相似的生长习惯。此外，还证明了暴露于细胞培养物后 PANI-PAAMPSA 薄膜的导电性，表明材料在细胞生长后仍保留其功能。这些结果表明，这种类型的模板合成的聚苯胺可以成功地用作功能性导电生物材料。
