Department of Biomedical Engineering, Ohio State University, Columbus, OH 43210, USA.
Macromol Rapid Commun. 2011 Jun 16;32(12):887-92. doi: 10.1002/marc.201100095. Epub 2011 May 17.
We report here that by good design, polyaniline (PANI) can be cytocompatible and formed into usable scaffolds for bio-medical applications. By adjusting the ratio of two monomers, aniline (AN) and metanilic acid (MA), a series of copolymers with different sulfonation degrees have been synthesized. Four-probe conductivity measurements showed that as the sulfonation degree increased, the conductivity decreased. XPS analysis was used to determine the sulfur/nitrogen ratio. In vitro cell culture study was conducted with human osteosarcoma (HOS) cells. Microscopic observation did not show abnormal cellular behavior when sulfonated polyaniline (SPAN) was put in direct contact with HOS cells. Cells growing on the non-transparent dark green SPAN films were observed with fluorescence by laser scanning cytometry (LSC). In proliferation studies more than 70% of cells were found viable on SPAN compared to 88% for poly(L-lactic acid) with the number of cells growing on glass as a control, indicating generally good biocompatibility. We expect these polymers would have great potential in biological applications of conducting polymers as we determine that a variety of physical and chemical properties can be controlled through synthesis.
我们在此报告，通过良好的设计，聚苯胺（PANI）可以具有细胞相容性，并形成可用于生物医学应用的有用支架。通过调整两种单体苯胺（AN）和间苯二甲酸（MA）的比例，已经合成了一系列具有不同磺化度的共聚物。四探针电导率测量表明，随着磺化度的增加，电导率降低。XPS 分析用于确定硫/氮比。通过人骨肉瘤（HOS）细胞进行体外细胞培养研究。当磺化聚苯胺（SPAN）与 HOS 细胞直接接触时，显微镜观察没有显示出异常的细胞行为。用激光扫描共聚焦显微镜（LSC）观察生长在不透明深绿色 SPAN 薄膜上的细胞。在增殖研究中，与对照组玻璃上生长的细胞相比，SPAN 上有超过 70%的细胞具有活力，而聚（L-乳酸）上有 88%的细胞具有活力，表明其具有良好的生物相容性。我们期望这些聚合物在导电聚合物的生物应用中具有巨大的潜力，因为我们确定可以通过合成来控制各种物理和化学性质。
