Rejmontová Petra, Capáková Zdenka, Mikušová Nikola, Maráková Nela, Kašpárková Věra, Lehocký Marián, Humpolíček Petr
Centre of Polymer Systems, Tomas Bata University in Zlín, třída Tomáše Bati 5678, 760 01 Zlín, Czech Republic.
Int J Mol Sci. 2016 Sep 15;17(9):1439. doi: 10.3390/ijms17091439.
Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.
聚苯胺因其固有的导电性和与天然组织极为相似的材料特性，在生物医学领域展现出巨大潜力并有望得到广泛应用。表面性质至关重要，因为它们预先决定了与生物流体、蛋白质和细胞的任何相互作用。聚苯胺的一个优点是其表面易于改性，例如通过使用各种掺杂酸。对小鼠胚胎成纤维细胞在原始聚苯胺薄膜以及掺杂氨基磺酸和磷钨酸的薄膜上的粘附、增殖和迁移进行了研究。此外，还测试了以不同比例添加聚（2-丙烯酰胺基-2-甲基-1-丙烷磺酸）的聚苯胺薄膜。结果表明，NIH/3T3细胞系能够在原始聚苯胺薄膜以及掺杂氨基磺酸和磷钨酸的薄膜上粘附、增殖和迁移；因此，在生物医学中使用上述形式似乎很有前景。然而，加入聚（2-丙烯酰胺基-2-甲基-1-丙烷磺酸）改变了聚苯胺薄膜的表面性质，并显著影响细胞行为。为了揭示影响表面/细胞相互作用的关键因素，在单个样品表面能的背景下讨论了细胞行为。清楚地表明，样品和细胞的表面能之间的差异越小，表面的细胞相容性就越高。
