College of Food Science and Nutritional Engineering, China Agricultural University, China.
Int J Food Microbiol. 2010 May 15;139(3):177-81. doi: 10.1016/j.ijfoodmicro.2010.02.029. Epub 2010 Mar 6.
In this paper, the ultrasonic inactivation efficacy of Alicyclobacillus acidiphilus DSM14558(T) and Alicyclobacillus acidoterrestris DSM 3922(T) inoculated into apple juice was investigated at power level from 200 to 600 W for treatment time from 1 to 30 min. The survival ratio of A. acidiphilus DSM14558(T) and A. acidoterrestris DSM 3922(T) decreased with the time of exposure to ultrasounds and with their power. Weibull distribution function, log-logistic model, modified Gompertz equation and biphasic linear model were used to describe the experimental data and the fitness of the models was assessed by the adjusted correlation coefficient (adj-R(2)) and the root mean square error (RMSE). The results showed that, for A. acidiphilus DSM14558(T), the Weibull distribution function described well the characteristic of ultrasonic inactivation, while for A. acidoterrestris DSM 3922(T), the adequate one was the biphasic linear model. Alicyclobacilli had a much higher resistance to ultrasonic treatments in apple juice than in buffer, which indicated that the resistance of alicyclobacilli to ultrasound varied significantly depending on their environment; A. acidoterrestris DSM 3922(T), with the greatest microbial reduction of 4.56 log cycles at 600 W for 30 min, seemed more sensitive to ultrasonic treatments than A. acidiphilus DSM14558(T).
本文研究了 200-600 W 功率下处理 1-30 min 对苹果汁中嗜酸耐热菌（Alicyclobacillus acidiphilus DSM14558(T)）和嗜酸耐热杆菌（Alicyclobacillus acidoterrestris DSM 3922(T)）的超声灭活效果。A. acidiphilus DSM14558(T)和 A. acidoterrestris DSM 3922(T)的存活比例随超声暴露时间和功率的增加而降低。威布尔分布函数、双对数模型、修正的 Gompertz 方程和双相线性模型用于描述实验数据，通过调整相关系数（adj-R(2)）和均方根误差（RMSE）评估模型的拟合程度。结果表明，对于 A. acidiphilus DSM14558(T)，威布尔分布函数很好地描述了超声灭活的特征，而对于 A. acidoterrestris DSM 3922(T)，合适的模型是双相线性模型。嗜酸耐热菌在苹果汁中的超声处理抗性明显高于缓冲液，表明嗜酸耐热菌对超声的抗性随环境的变化而显著变化；在 600 W 处理 30 min 时，A. acidoterrestris DSM 3922(T)的微生物减少量最大，为 4.56 log 循环，比 A. acidiphilus DSM14558(T)更敏感。
