Ferrario Mariana I, Guerrero Sandra N
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, CABA, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Buenos Aires, Argentina.
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, CABA, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina, Buenos Aires, Argentina.
Rev Argent Microbiol. 2018 Jan-Mar;50(1):3-11. doi: 10.1016/j.ram.2017.04.002. Epub 2017 Jul 17.
The purpose of this study was to analyze the response of different initial contamination levels of Alicyclobacillus acidoterrestris ATCC 49025 spores in apple juice as affected by pulsed light treatment (PL, batch mode, xenon lamp, 3pulses/s, 0-71.6J/cm). Biphasic and Weibull frequency distribution models were used to characterize the relationship between inoculum size and treatment time with the reductions achieved after PL exposure. Additionally, a second order polynomial model was computed to relate required PL processing time to inoculum size and requested log reductions. PL treatment caused up to 3.0-3.5 log reductions, depending on the initial inoculum size. Inactivation curves corresponding to PL-treated samples were adequately characterized by both Weibull and biphasic models (R 94-96%), and revealed that lower initial inoculum sizes were associated with higher inactivation rates. According to the polynomial model, the predicted time for PL treatment increased exponentially with inoculum size.
本研究的目的是分析脉冲光处理（PL，间歇模式，氙灯，3脉冲/秒，0 - 71.6 J/cm²）对苹果汁中不同初始污染水平的嗜酸耐热脂肪芽孢杆菌ATCC 49025孢子的影响。采用双相和威布尔频率分布模型来表征接种量与处理时间之间的关系以及脉冲光处理后实现的对数减少量。此外，计算了二阶多项式模型，以将所需的脉冲光处理时间与接种量和要求的对数减少量相关联。根据初始接种量的不同，脉冲光处理可导致高达3.0 - 3.5个对数减少量。威布尔模型和双相模型都能很好地表征脉冲光处理样品的失活曲线（R² = 94 - 96%），并且表明较低的初始接种量与较高的失活率相关。根据多项式模型，脉冲光处理的预测时间随接种量呈指数增加。
