Departamento de Oftalmologia e Otorrinolaringologia, UFMG, Brazil.
Braz J Otorhinolaryngol. 2010 Jan-Feb;76(1):25-8. doi: 10.1590/S1808-86942010000100005.
Although the cytokine profile in nasal polyposis is well documented, little is known about cytokines associated to cystic fibrosis.
Assess the expression of cytokines IL not4, IL not5, IL not6, IL not8, GM not-CSF and IFN not-y, analyzed through RT-PCR, in the polyps of patients with cystic fibrosis.
A cross-sectional, prospective study was carried out with 24 patients, 13 of whom had cystic fibrosis and nasal polyposis (Cystic Fibrosis Group) and 11 had normal otorhinolaryngological exams (Control Group). The average age was 21 years (3 not57); 12 participants were males and 12 were females. The cytokine profile was studied in mucosal fragments (Control Group) or nasal polyps (Cystic Fibrosis Group) through RT notPCR. Transcriptions were studied for cytokines IL not4, IL not5, IL not6, IL not8, IFN noty and GM notCSF, adjusted for the beta not-actin value.
Interleukins 5, 6, 8 and GM notCSF were similar in both groups (p>0.05). There were lower values of IFN-y not (p=0.03) and a strong tendency toward an increase in IL not4 (p=0.06) in the Cystic Fibrosis Group.
Inflammatory and structural cells may produce messenger RNA for IL not4, blocking the production of other cytokines such as IFN-y, suggesting the participation of this mechanism in the formation of polyps in cystic fibrosis.
通过 RT-PCR 分析，评估囊性纤维化患者鼻息肉中细胞因子 IL-4、IL-5、IL-6、IL-8、GM-CSF 和 IFN-y 的表达。
这是一项横断面、前瞻性研究，共纳入 24 名患者，其中 13 名患有囊性纤维化和鼻息肉（囊性纤维化组），11 名患者的耳鼻喉检查正常（对照组）。平均年龄为 21 岁（35-77 岁）；12 名参与者为男性，12 名参与者为女性。通过 RT-PCR 研究对照组的黏膜片段或囊性纤维化组的鼻息肉中的细胞因子谱。对细胞因子 IL-4、IL-5、IL-6、IL-8、IFN-y 和 GM-CSF 的转录进行研究，通过 β-肌动蛋白值进行调整。
两组间白细胞介素 5、6、8 和 GM-CSF 相似（p>0.05）。囊性纤维化组 IFN-y 的表达较低（p=0.03），IL-4 的表达呈增加趋势（p=0.06）。
炎症和结构细胞可能产生 IL-4 的信使 RNA，从而阻断 IFN-y 等其他细胞因子的产生，提示这种机制参与了囊性纤维化中息肉的形成。
