Rosenberg G H, Alahari S K, Käufer N F
Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104.
Mol Gen Genet. 1991 Apr;226(1-2):305-9. doi: 10.1007/BF00273617.
We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.
我们构建了一组温度敏感型（ts）粟酒裂殖酵母突变株库。约150个此类突变体用含有一个人工内含子的ura4基因进行转化。我们筛选这些ts突变体，以寻找ura4内含子剪接缺陷的突变体。通过这种方法，分离出了三个在剪接过程中存在普遍缺陷的突变体。其中两个突变体属于prp1互补群，另一个则定义了一个新的互补群，即prp4。
