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通过横向非均匀场捕获的自由流动电泳。

Free-flow electrophoresis with trapping by a transverse inhomogeneous field.

作者信息

Ajdari A, Prost J

机构信息

Laboratoire de Physico-Chimie Théorique, Centre National de la Recherche Scientifique, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris, France.

出版信息

Proc Natl Acad Sci U S A. 1991 May 15;88(10):4468-71. doi: 10.1073/pnas.88.10.4468.

DOI:10.1073/pnas.88.10.4468
PMID:2034684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC51681/
Abstract

We propose an electrophoretic technique combining the use of a series of dielectric traps controlled by an ac electric field and conventional continuous field free-flow electrophoresis. From the theoretical model that we describe, one can expect, for DNA electrophoresis, an improvement of one to two orders of magnitude in the selectivities or experimental durations.

摘要

我们提出了一种电泳技术,该技术结合了一系列由交流电场控制的介电阱的使用和传统的连续无场自由流动电泳。从我们所描述的理论模型来看,对于DNA电泳,可以预期在选择性或实验持续时间方面提高一到两个数量级。

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Proc Natl Acad Sci U S A. 1991 May 15;88(10):4468-71. doi: 10.1073/pnas.88.10.4468.
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本文引用的文献

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Size flucuations can explain anomalous mobility in field-inversion electrophoresis of DNA.
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Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
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Pulsed-field gel electrophoresis of very large DNA molecules.超大DNA分子的脉冲场凝胶电泳
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Fractionation of macromolecules in an alternating transverse electric field: simulation of the method.在交变横向电场中对大分子进行分级分离:该方法的模拟
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