Department of Food Engineering, URI-Campus de Erechim, Av. Sete de Setembro 1621, Erechim 99700-000, RS, Brazil.
J Biotechnol. 2010 May 17;147(2):108-15. doi: 10.1016/j.jbiotec.2010.03.014. Epub 2010 Mar 27.
This work reports new experimental data and mathematical modeling of lipase-catalyzed biodiesel production using soybean oil and ethanol as substrates and pressurized n-propane as solvent. The experiments were carried out in a batch reactor, recording the reaction kinetics and evaluating the effects of temperature in the range of 45-70 degrees C, enzyme content from 1 to 20 wt% and oil to ethanol molar ratios of 1:3, 1:6, 1:9 and 1:15. The solvent to substrates mass ratio and pressure were set at 2:1 and 50 bar, respectively. Results showed that lipase-catalyzed alcoholysis in propane medium might be a potential alternative to conventional techniques for biodiesel production, since good conversions were obtained at mild temperature and pressure conditions. The semi-empirical mathematical model based on balance equations, adopted to describe the transesterification kinetics in pressurized n-propane, yielded relative deviations between experimental and calculated values lower than 10%, thus allowing a satisfactory representation of experimental results and a better understanding of the transesterification reaction.
这项工作报道了使用大豆油和乙醇作为底物，加压正丙烷作为溶剂，通过脂肪酶催化生产生物柴油的新实验数据和数学建模。实验在间歇式反应器中进行，记录了反应动力学，并评估了温度在 45-70°C 范围内、酶含量在 1-20wt%之间以及油与乙醇摩尔比为 1:3、1:6、1:9 和 1:15 的影响。溶剂与底物的质量比和压力分别设定为 2:1 和 50 巴。结果表明，在丙烷介质中脂肪酶催化的醇解可能是生物柴油生产的传统技术的一种潜在替代方法，因为在温和的温度和压力条件下可以获得良好的转化率。基于平衡方程的半经验数学模型，用于描述加压正丙烷中的酯交换动力学，实验值与计算值之间的相对偏差低于 10%，从而可以对实验结果进行令人满意的表示，并更好地理解酯交换反应。
