Department of Engineering Modelling, University of Calabria, via P. Bucci Cubo 45/A, Arcavacata di Rende (CS), Italy.
Bioprocess Biosyst Eng. 2010 Aug;33(6):701-10. doi: 10.1007/s00449-009-0392-z. Epub 2009 Nov 10.
In this paper, the reaction of enzymatic trans-esterification of glycerides with ethanol in a reaction medium containing hexane at a temperature of 37 degrees C has been studied. The enzyme was Lipase from Mucor miehei, immobilized on ionic exchange resin, aimed at achieving high catalytic specific surface and recovering, regenerating and reusing the biocatalyst. A kinetic analysis has been carried out to identify the reaction path; the rate equation and kinetic parameters have been also calculated. The kinetic model has been validated by comparison between predicted and experimental results. Mass transport resistances estimation was undertaken in order to verify that the kinetics found was intrinsic. Model potentialities in terms of reactors design and optimization are also shown.
本文研究了在 37°C 的温度下，含有己烷的反应介质中甘油酯与乙醇的酶促转酯化反应。酶是米黑毛霉固定在离子交换树脂上的脂肪酶，旨在实现高催化比表面积并回收、再生和再利用生物催化剂。进行了动力学分析以确定反应途径；还计算了速率方程和动力学参数。通过比较预测值和实验值验证了动力学模型。为了验证发现的动力学是本征的，进行了传质阻力估计。还展示了模型在反应器设计和优化方面的潜力。
