Marecki N, Becker F, Baca O G, Paretsky D
Infect Immun. 1978 Jan;19(1):272-80. doi: 10.1128/iai.19.1.272-280.1978.
Changes in plasma membrane proteins of guinea pig liver and L-929 cells were studied during infection with Coxiella burnetii. Polypeptide species resolved by disc polyacrylamide gel electrophoresis with sodium dodecyl sulfate showed quantitative but no qualitative differences between uninfected and infected samples. When the O'Farrell technique of isoelectric focusing, followed by sodium dodecyl sulfate-slab gel polyacrylamide gel electrophoresis, was employed, additional polypeptides were resolved. Livers and L cells were labeled with [3H]-glucosamine. Infected livers incorporated less [3H]glucosamine in the membrane proteins than uninfected material, presumably indicating lower glycoprotein levels. Infected L-cell membranes incorporated greater amounts of [3H]glucosamine, and also were labeled to a greater extent than uninfected membranes, employing the [125I]lactoperoxidase technique. Uninfected L cells showed a greater agglutinability with concanavalin A than did infected cells. Infected livers had much greater levels of cyclic adenosine 3',5'-monophosphate. The data indicate changes in plasma membranes as a result of infection. Possible physiological consequences of membrane changes are discussed.
在感染伯纳特立克次体期间，对豚鼠肝脏和L - 929细胞的质膜蛋白变化进行了研究。用十二烷基硫酸钠圆盘聚丙烯酰胺凝胶电泳分离的多肽种类显示，未感染和感染样品之间存在数量差异，但无质量差异。当采用奥法雷尔等电聚焦技术，随后进行十二烷基硫酸钠平板凝胶聚丙烯酰胺凝胶电泳时，可分辨出更多的多肽。用[³H] - 葡糖胺标记肝脏和L细胞。感染的肝脏在膜蛋白中掺入的[³H] - 葡糖胺比未感染的材料少，这可能表明糖蛋白水平较低。采用[¹²⁵I]乳过氧化物酶技术，感染的L细胞膜掺入的[³H] - 葡糖胺量更多，且标记程度也比未感染的膜更高。未感染的L细胞与伴刀豆球蛋白A的凝集性比感染的细胞更高。感染的肝脏中3',5'-环磷酸腺苷的水平要高得多。数据表明感染导致质膜发生了变化。文中讨论了膜变化可能产生的生理后果。
