Mallavia L P, Paretsky D
J Bacteriol. 1967 May;93(5):1479-83. doi: 10.1128/jb.93.5.1479-1483.1967.
Protein synthesis, in terms of (14)C-labeled amino acid incorporation into a hot trichloroacetic acid fraction, was studied in cell-free preparations of Coxiella burnetii, and in uninfected and Q fever-infected guinea pig and chick embryo hosts. Purified and disrupted suspensions of C. burnetii incorporated (14)C-labeled l-leucine, l-phenylalanine and algal hydrolysate. Livers of infected guinea pigs and chick embryos had a greater incorporation rate at the height of infection than comparable preparations from uninfected animals. As chick embryonic development continued during infection, the rate of incorporation progressively decreased below that of uninfected embryos.
就（14）C标记的氨基酸掺入热的三氯乙酸部分而言，在伯氏考克斯氏体的无细胞制剂以及未感染和感染Q热的豚鼠和鸡胚宿主中研究了蛋白质合成。纯化并破碎的伯氏考克斯氏体悬浮液掺入了（14）C标记的L-亮氨酸、L-苯丙氨酸和藻类水解产物。感染豚鼠和鸡胚的肝脏在感染高峰期的掺入率高于未感染动物的相应制剂。随着感染期间鸡胚发育的继续，掺入率逐渐下降至低于未感染胚胎的水平。
