Divison of Orthodontics and Dentofacial Deformities, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi 110029, India.
Centre for Biomedical Engineering, Indian Institute of Technology, Hauz Khas, New Delhi 110016, India; Department of Biomedical Engineering, All India Institute of Medical Sciences, New Delhi 110029, India.
Colloids Surf B Biointerfaces. 2018 Jan 1;161:1-9. doi: 10.1016/j.colsurfb.2017.10.024. Epub 2017 Oct 9.
Surface modification of medical grade V titanium alloy (Ti-6Al-4V) with biomolecules is an important and vital step for tailoring it for various biomedical applications. Present study investigates theinfluence of type I human collagen (T1HC) bio-conjugation through a three stage process. Polished grade V titanium alloy discs were functionalizedwith free OH group by means of controlled heat and alkali treatment followed by coating of 3-aminopropyltriethoxy (APTES) silane couplingagent. T1HC were bio-conjugated through 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride N-hydroxysuccinimide (EDCNHS)coupling reaction. At each stage, grade V titanium alloy surfaces were characterized by atomic force microscopy (AFM), scanning electronmicroscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Xrayphotoelectron spectroscopy (XPS). FTIR and XPS studies confirms thecovalent attachment of APTES with titanium alloy surface while terminalamine groups of APTES remained free for further attachment of T1HCthrough covalent bond. Aqueous stability of bio-conjugated titanium discsat various pH and time intervals (i.e. at pH of 5.5, 6.8 and 8.0 at timeinterval of 27 and 48h) confirmed the stability of T1HC bioconjugated collagen on titanium surface. Further human periodontalfibroblast cell line (HPdlF) culture revealed enhanced adhesion on theT1HC bio-conjugated surface compared to the polystyrene and polishedgrade V titanium alloy surfaces.
医用级 V 型钛合金（Ti-6Al-4V）表面的生物分子修饰是为各种生物医学应用定制其性能的重要步骤。本研究通过三个阶段的过程研究了 I 型人胶原蛋白（T1HC）的生物共轭作用的影响。通过控制热和碱处理使抛光的 V 型钛合金盘具有游离的 OH 基团，然后用 3-氨丙基三乙氧基（APTES）硅烷偶联剂进行涂层。通过 1-乙基-3-（3-二甲基氨基丙基）碳化二亚胺盐酸盐 N-羟基琥珀酰亚胺（EDCNHS）偶联反应将 T1HC 生物共轭。在每个阶段，V 型钛合金表面都通过原子力显微镜（AFM）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）进行了表征。FTIR 和 XPS 研究证实了 APTES 与钛合金表面的共价结合，而 APTES 的末端胺基仍然游离，可通过共价键进一步与 T1HC 结合。在不同的 pH 值和时间间隔（即在 pH 值为 5.5、6.8 和 8.0 时，时间间隔为 27 和 48h）下，生物结合钛盘的水稳定性证实了钛表面 T1HC 生物结合胶原的稳定性。进一步的人牙周成纤维细胞系（HPdlF）培养表明，与聚苯乙烯和抛光的 V 型钛合金表面相比，T1HC 生物结合表面的粘附性增强。
