Instituto del Frío (CSIC), José Antonio Novais 10, Ciudad Universitaria s/n, 28040 Madrid, Spain.
J Chromatogr A. 2010 Apr 30;1217(18):3063-6. doi: 10.1016/j.chroma.2010.02.073. Epub 2010 Mar 3.
An improved HPLC-ELSD method has been developed for the analysis of the lipid classes of buttermilk and milk from different species, focused in the phospholipids fraction without a prior fractionation step and in a single run. The total lipid profile analysis showed the major and minor lipid compounds as cholesterol esters, triacylglycerides, cholesterol, diacylglycerides, free fatty acids, monoacylglycerides, and also the polar compounds as glucosylceramide, lactosylceramide, phosphatidyl-ethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin and lysophosphatidylcholine. The identification and quantification of the different compounds, using calibration curves made with individual standards and the low coefficients of variation obtained in the inter- and intra-assays showed the suitability of the developed method. In this study, we optimized and validated a quantitative HPLC-ELSD method at a concentration level suitable for routine analysis of the major lipid classes in milk and dairy products.
已经开发出一种改进的 HPLC-ELSD 方法，用于分析来自不同物种的黄油和牛奶的脂质类，重点是磷脂部分，无需进行预先的分级步骤，且在单次运行中完成。总脂质谱分析显示了主要和次要脂质化合物，如胆固醇酯、三酰基甘油、胆固醇、二酰基甘油、游离脂肪酸、单酰基甘油，以及极性化合物，如葡糖神经酰胺、乳酰神经酰胺、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰胆碱、鞘磷脂和溶血磷脂酰胆碱。使用单独的标准品制作校准曲线，并在间和内测定中获得低变异系数，对不同化合物进行鉴定和定量，表明所开发方法的适用性。在这项研究中，我们优化和验证了一种适用于常规分析牛奶和乳制品中主要脂质类的定量 HPLC-ELSD 方法，其浓度水平适合于常规分析。
