Laboratory of Parasitology, Statens Serum Institut, Copenhagen, Denmark.
Int J Parasitol. 2010 Jun;40(7):775-8. doi: 10.1016/j.ijpara.2010.03.003. Epub 2010 Mar 30.
Six ssrRNA gene sequences were obtained by PCR amplification of DNA from uninucleated Entamoeba cysts isolated from fresh faeces of sheep, cows, a roe deer and a reindeer. Phylogenetic analysis using sequences of non-, uni-, quadri- and octonucleate cyst-producing Entamoeba spp. for comparison showed that all six isolates formed a separate clade nested within the clade of quadrinucleate cyst producers. The data indicate that Entamoeba bovis can be isolated from ruminant hosts other than cattle, and we suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis.
从绵羊、牛、狍和驯鹿的新鲜粪便中分离的单核包囊 Entamoeba 中提取 DNA，通过 PCR 扩增获得了 6 个 ssrRNA 基因序列。对非、单核、四核和八核包囊产生 Entamoeba spp. 的序列进行系统发育分析表明，所有 6 个分离株均形成一个单独的分支，嵌套在四核包囊产生者的分支内。这些数据表明，除了牛以外，牛艾美球虫还可以从反刍动物宿主中分离出来，我们建议将与本研究中分析的绵羊和牛分离株聚类的生物命名为牛艾美球虫。
