• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

中国安徽省圈养梅花鹿体内牛内阿米巴的首次检测与分子鉴定

First Detection and Molecular Identification of Entamoeba bovis in Farm-Raised Sika Deer from Anhui Province, China.

作者信息

Liu Xin-Chao, Ren Qi, Guo Jie, Chen Dong-Qian, Li Qiao-Qiao, Luo Xin-Yu, Gu You-Fang, Li Wen-Chao

机构信息

Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, College of Animal Science, Anhui Science and Technology University, Fengyang, Chuzhou, 233100, China.

Animal Husbandry Development Center of Lu'an City, Lu'an, 237000, China.

出版信息

Acta Parasitol. 2022 Dec;67(4):1782-1787. doi: 10.1007/s11686-022-00610-9. Epub 2022 Aug 26.

DOI:10.1007/s11686-022-00610-9
PMID:36018470
Abstract

BACKGROUND

Protozoans of Entamoeba spp. are one of the most common enteric parasites that infect humans and diverse animals including deer.

PURPOSE

However, data regarding the prevalence and species/genotypes of Entamoeba spp. in deer in China is scarce. This study investigated the prevalence and species distribution of Entamoeba spp. in sika deer (Cervus nippon) in Anhui Province.

METHODS

In our survey, 336 fecal samples were collected from five sika deer farms in different regions of Anhui Province. All samples were examined for the presence of Entamoeba spp. by PCR and phylogenetic analysis of the conserved 18S rRNA gene.

RESULTS

106/336 (31.5%) fecal samples were positive for Entamoeba spp. A statistically significant difference in the prevalence of Entamoeba spp. infection was observed between sampling farms (p < 0.001), and the prevalence of Entamoeba spp. in male and female sika deer showed no significant difference (p > 0.05). Sequence and phylogenetic analysis revealed the single species, E. bovis, was identified in this study.

CONCLUSION

This is the first report about the identification of E. bovis in farm-raised sika deer in China, and these results expand our understanding of host range and species distribution of Entamoeba spp. in ruminants.

摘要

背景

内阿米巴属原生动物是最常见的肠道寄生虫之一，可感染人类及包括鹿在内的多种动物。

目的

然而，关于中国鹿体内内阿米巴属的流行情况及种类/基因型的数据稀缺。本研究调查了安徽省梅花鹿（Cervus nippon）体内内阿米巴属的流行情况及种类分布。

方法

在我们的调查中，从安徽省不同地区的五个梅花鹿养殖场采集了336份粪便样本。通过PCR及对保守的18S rRNA基因进行系统发育分析，检测所有样本中是否存在内阿米巴属。

结果

106/336（31.5%）份粪便样本内阿米巴属呈阳性。在各采样养殖场之间，内阿米巴属感染率存在统计学显著差异（p < 0.001），且雄性和雌性梅花鹿的内阿米巴属感染率无显著差异（p > 0.05）。序列和系统发育分析显示，本研究中鉴定出单一物种——牛内阿米巴。

结论

这是中国首次关于养殖场养殖梅花鹿体内牛内阿米巴鉴定的报告，这些结果拓展了我们对反刍动物体内内阿米巴属宿主范围及种类分布的认识。

相似文献

1
First Detection and Molecular Identification of Entamoeba bovis in Farm-Raised Sika Deer from Anhui Province, China.中国安徽省圈养梅花鹿体内牛内阿米巴的首次检测与分子鉴定
Acta Parasitol. 2022 Dec;67(4):1782-1787. doi: 10.1007/s11686-022-00610-9. Epub 2022 Aug 26.
2
First Evidence of Parasites in Australian Wild Deer and Assessment of Transmission to Cattle.澳大利亚野生鹿群中寄生虫的首次证据及其向牛传播的评估。
Front Cell Infect Microbiol. 2022 Jun 10;12:883031. doi: 10.3389/fcimb.2022.883031. eCollection 2022.
3
Molecular detection of Blastocystis in black bears and sika deer in northern China.中国北方黑熊和梅花鹿中 Blastocystis 的分子检测。
Parasitol Res. 2021 Apr;120(4):1481-1487. doi: 10.1007/s00436-021-07068-0. Epub 2021 Feb 4.
4
Molecular detection and identification of piroplasms in sika deer (Cervus nippon) from Jilin Province, China.中国吉林省梅花鹿（Cervus nippon）中梨形虫的分子检测与鉴定
Parasit Vectors. 2016 Mar 16;9:156. doi: 10.1186/s13071-016-1435-3.
5
Molecular detection of Cryptosporidium and Enterocytozoon bieneusi in dairy calves and sika deer in four provinces in Northern China.在中国北方四省的奶牛犊和梅花鹿中检测隐孢子虫和肠上皮细胞内原虫
Parasitol Res. 2020 Jan;119(1):105-114. doi: 10.1007/s00436-019-06498-1. Epub 2019 Nov 26.
6
Identification and molecular survey of Borrelia burgdorferi sensu lato in sika deer (Cervus nippon) from Jilin Province, north-eastern China.中国东北地区吉林省梅花鹿（Cervus nippon）中伯氏疏螺旋体狭义种的鉴定与分子调查。
Acta Trop. 2017 Feb;166:54-57. doi: 10.1016/j.actatropica.2016.11.002. Epub 2016 Nov 3.
7
Detection and identification of Theileria infection in sika deer ( Cervus nippon ) in China.中国梅花鹿（Cervus nippon）泰勒虫感染的检测与鉴定。
J Parasitol. 2012 Jun;98(3):598-603. doi: 10.1645/JP-GE-2883.1.
8
The first survey and molecular identification of Entamoeba spp. in farm animals on Qinghai-Tibetan Plateau of China.中国青藏高原地区家畜内阿米巴属的首次调查和分子鉴定。
Comp Immunol Microbiol Infect Dis. 2021 Apr;75:101607. doi: 10.1016/j.cimid.2020.101607. Epub 2020 Dec 24.
9
Molecular diversity of rumen bacterial communities from tannin-rich and fiber-rich forage fed domestic Sika deer (Cervus nippon) in China.中国富含单宁和纤维的饲草饲养的梅花鹿瘤胃细菌群落的分子多样性。
BMC Microbiol. 2013 Jul 8;13:151. doi: 10.1186/1471-2180-13-151.
10
Mycobacterium tuberculosis and M. bovis infection in Feedlot Deer (Cervus unicolor swinhoei and C. nippon taiouanus) in Taiwan.台湾饲养鹿（台湾梅花鹿和日本梅花鹿）中的结核分枝杆菌和牛分枝杆菌感染。
J Microbiol Immunol Infect. 2012 Dec;45(6):426-34. doi: 10.1016/j.jmii.2011.12.022. Epub 2012 May 10.

引用本文的文献

1
First report on the infection of and in the endangered Chinese Milu deer () in China.中国濒危麋鹿（）感染和的首次报告。 （注：原文中“ and ”处信息缺失，不太完整准确）
One Health. 2025 Jun 13;21:101112. doi: 10.1016/j.onehlt.2025.101112. eCollection 2025 Dec.
2
First Molecular Identification of spp. in Sheep, Beef Cattle, and Dairy Cattle in Shanxi Province, North China.中国北方山西省绵羊、肉牛和奶牛中 spp. 的首次分子鉴定
Vet Sci. 2025 Jan 4;12(1):19. doi: 10.3390/vetsci12010019.
3
Unusual Findings of Human-Associated Four-Nucleated Species in Captive Wild Animals.

本文引用的文献

1
First Evidence of Parasites in Australian Wild Deer and Assessment of Transmission to Cattle.澳大利亚野生鹿群中寄生虫的首次证据及其向牛传播的评估。
Front Cell Infect Microbiol. 2022 Jun 10;12:883031. doi: 10.3389/fcimb.2022.883031. eCollection 2022.
2
First report on the molecular detection of Entamoeba bovis from the endangered wild water buffalo (Bubalus arnee) in Nepal.首次报道从尼泊尔濒危野生水牛（Bubalus arnee）中检测到的肠内变形虫（Entamoeba bovis）。
Vet Med Sci. 2022 Mar;8(2):799-807. doi: 10.1002/vms3.697. Epub 2021 Dec 17.
3
Epidemiology, species composition and genetic diversity of tetra- and octonucleated Entamoeba spp. in different Brazilian biomes.
圈养野生动物中人类相关四核物种的异常发现。
Animals (Basel). 2025 Jan 3;15(1):90. doi: 10.3390/ani15010090.
巴西不同生物群落中四核和八核内阿米巴的流行病学、物种组成和遗传多样性。
Parasit Vectors. 2021 Mar 17;14(1):160. doi: 10.1186/s13071-021-04672-y.
4
Occurrence of gastro-intestinal parasites among small ruminants in Malaysia: highlighting Dicrocoelium infection in goats.马来西亚小型反刍动物胃肠道寄生虫的发生情况：重点关注山羊的双腔吸虫感染
Trop Biomed. 2017 Dec 1;34(4):963-969.
5
The first survey and molecular identification of Entamoeba spp. in farm animals on Qinghai-Tibetan Plateau of China.中国青藏高原地区家畜内阿米巴属的首次调查和分子鉴定。
Comp Immunol Microbiol Infect Dis. 2021 Apr;75:101607. doi: 10.1016/j.cimid.2020.101607. Epub 2020 Dec 24.
6
First Detection and Molecular Identification of Entamoeba in Yaks from China.中国牦牛体内溶组织内阿米巴的首次检测与分子鉴定
Acta Parasitol. 2021 Mar;66(1):264-270. doi: 10.1007/s11686-020-00258-3. Epub 2020 Aug 11.
7
Occurrence and genetic identifications of porcine Entamoeba, E. suis and E. polecki, at Tangerang in West Java, Indonesia.在印度尼西亚西爪哇的丹格朗发现了猪内阿米巴、E. suis 和 E. polecki，并进行了基因鉴定。
Parasitol Res. 2020 Sep;119(9):2983-2990. doi: 10.1007/s00436-020-06806-0. Epub 2020 Jul 24.
8
Prevalence and Genetic Identification of Three Species in Pigs in Southeastern China.中国东南部猪中三种虫种的流行情况和遗传鉴定。
Biomed Res Int. 2019 Nov 7;2019:2824017. doi: 10.1155/2019/2824017. eCollection 2019.
9
First detection and molecular identification of Entamoeba bovis from Japanese cattle.首次从日本牛中检测并分子鉴定出牛内阿米巴。
Parasitol Res. 2018 Jan;117(1):339-342. doi: 10.1007/s00436-017-5689-2. Epub 2017 Nov 28.
10
Molecular characterisation of protist parasites in human-habituated mountain gorillas (Gorilla beringei beringei), humans and livestock, from Bwindi impenetrable National Park, Uganda.来自乌干达布温迪难以穿越国家公园的与人类接触的山地大猩猩（山地大猩猩指名亚种）、人类和家畜体内原生生物寄生虫的分子特征分析
Parasit Vectors. 2017 Jul 18;10(1):340. doi: 10.1186/s13071-017-2283-5.