[用于快速检测四川结核分枝杆菌中利福平及异烟肼耐药性的基因分型MTBDRplus检测法]

[GenoType MTBDRplus assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis in Sichuan].

作者信息

Duo Li-na, Wang Lan-lan, Song Xing-bo, Xie Yi, Lu Xiao-jun, Fan Hong, Ying Bin-wu, Wang Ting-ting, Zhang Lei

机构信息

Department of Laboratory Medicine, Sichuan University, Chengdu, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2011 May;31(5):822-4.

PMID:21602133

Abstract

OBJECTIVE

To explore the molecular and epidemic characteristics of rifampin (RFP) and isoniazid (INH) resistance of mycobacterium tuberculosis (MTB) in Sichuan.

METHODS

GenoType reg; MTBDRplus Assay GTplus was used to examine 68 clinical isolates of MTB and 105 clinical specimens for mutations in rpoB, katG and inhA genes related to RFP and INH resistance.

RESULTS

Of the 151 valid tests obtained, 44 (29.14%) and 26 (17.22%) showed drug resistance and multidrug resistance, respectively. Resistance to RFP and INH was found in 21.85% (33/151) and 24.50% (37/151) of the samples, respectively. The most prevalent mutations were rpoB S531L, katG S315T1 and inhA C-15T. The multidrug resistance rate in the sputum specimens was significantly higher than that in the non-respiratory samples (19.35% vs 7.41%).

CONCLUSION

Drug-resistant, especially multidrug-resistant tuberculosis is highly prevalent in Sichuan. The multidrug-resistant bacteria most frequently show rpoB S531L combined with katG S315T1 mutations, suggesting the necessity of developing rapid clinical identification methods for drug-resistant MTB to control the spread of the resistant strains.

摘要

目的

探讨四川地区结核分枝杆菌（MTB）对利福平（RFP）和异烟肼（INH）耐药的分子及流行特征。

方法

采用GenoType reg; MTBDRplus检测试剂盒（GTplus）检测68株MTB临床分离株及105份临床标本中与RFP和INH耐药相关的rpoB、katG和inhA基因的突变情况。

结果

在151次有效检测中，分别有44例（29.14%）和26例（17.22%）显示耐药和多重耐药。样本中对RFP和INH的耐药率分别为21.85%（33/151）和24.50%（37/151）。最常见的突变是rpoB S531L、katG S315T1和inhA C-15T。痰标本中的多重耐药率显著高于非呼吸道标本（19.35%对7.41%）。