Programs in Oromaxillofacial Infection & Immunity and BK21 CLS, Seoul National University and Dental Research Institute, Seoul, Republic of Korea.
BMC Microbiol. 2010 Apr 6;10:101. doi: 10.1186/1471-2180-10-101.
Although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. The aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a Roche/454 Genome Sequencer FLX Titanium. In addition, the effect of Toll-like receptor (TLR) 2 deficiency on oral microbiota was evaluated.
Eight oral bacterial communities of wild-type (n = 4) and TLR2 knock-out (n = 4) C57BL/6 mice were characterized by analyzing 80,046 reads of 16S rRNA genes obtained by pyrosequencing. Excluding the PCR primers, the average length of each sequencing product was 443 bp. The average species richness of the murine oral bacterial communities was estimated to be about 200, but the communities were dominated by only two main phyla and several species. Therefore, the bacterial communities were relatively simple. The bacterial composition of the murine oral microbiota was significantly different from that of humans, and the lack of TLR2 had a negligible effect on the murine oral microbiota.
Pyrosequencing using the Roche/454 FLX Titanium successfully characterized mouse oral bacterial communities. The relatively simple oral bacterial communities of mice were not affected by TLR2 deficiency. These findings will provide a basis for future studies on the role of periodontal pathogens in the murine model of periodontitis.
尽管小鼠长期以来一直被用作牙周炎的动物模型,但有关其固有口腔微生物群组成的信息有限。本研究的目的是通过使用最新的模型焦磷酸测序仪(罗氏/454 Genome Sequencer FLX Titanium)进行广泛的平行焦磷酸测序,来描述小鼠口腔细菌菌群。此外,还评估了 Toll 样受体(TLR)2 缺乏对口腔微生物群的影响。
通过对 80046 条 16S rRNA 基因焦磷酸测序获得的读取进行分析,对 8 种野生型(n = 4)和 TLR2 敲除(n = 4)C57BL/6 小鼠的口腔细菌群落进行了特征描述。排除 PCR 引物后,每个测序产物的平均长度为 443bp。估计小鼠口腔细菌群落的平均物种丰富度约为 200,但群落仅由两个主要门和几个物种主导。因此,细菌群落相对简单。鼠口腔微生物群的细菌组成与人类明显不同,TLR2 的缺乏对鼠口腔微生物群几乎没有影响。
使用罗氏/454 FLX Titanium 的焦磷酸测序成功地描述了小鼠口腔细菌群落。TLR2 缺乏对小鼠相对简单的口腔细菌群落没有影响。这些发现将为牙周病病原体在牙周炎小鼠模型中的作用的未来研究提供基础。