恒河猴或绿猴巨细胞病毒感染对未处理及经5-碘-2'-脱氧尿苷阻滞的细胞中DNA合成的影响。
Effect of rhesus or vervet cytomegalovirus infection of DNA synthesis in untreated and 5-iodo-2'-deoxyuridine-arrested cells.
作者信息
Waner J L, Budnick J E, Albrecht T B
出版信息
J Virol. 1978 Feb;25(2):465-70. doi: 10.1128/JVI.25.2.465-470.1978.
Abstract
Infection of permissive cells with either rhesus or vervet cytomegalovirus resulted in suppression of cellular DNA synthesis, only viral DNA was resolved by isopycnic centrifugation after 24 h postinoculation. Infection of 5-iodo-2'-deoxyuridine (IUdR)-arrested cells with either of the simian cytomegaloviruses, however, induced synthesis of cellular DNA of normal density; synthesis of cellular DNA substituted with IUdR as evidenced by resolution of a heavy DNA peak after isopycnic centrifugation was not observed. Stimulation of DNA synthesis in IUdR-arrested cells was not observed with virus inactivated with UV light.
摘要
用恒河猴或绿猴巨细胞病毒感染允许性细胞会导致细胞DNA合成受到抑制，接种后24小时通过等密度离心仅能分离出病毒DNA。然而，用任何一种猿猴巨细胞病毒感染经5-碘-2'-脱氧尿苷（IUdR）阻断的细胞，都会诱导正常密度的细胞DNA合成；未观察到经等密度离心后出现重DNA峰所证明的、被IUdR取代的细胞DNA合成。用紫外线灭活的病毒未观察到对IUdR阻断的细胞中DNA合成的刺激作用。
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