用于检测生殖支原体的聚合酶链反应的开发与评估

Development and evaluation of the polymerase chain reaction to detect Mycoplasma genitalium.

作者信息

Palmer H M, Gilroy C B, Furr P M, Taylor-Robinson D

机构信息

Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, U.K.

出版信息

FEMS Microbiol Lett. 1991 Jan 15;61(2-3):199-203. doi: 10.1016/0378-1097(91)90551-k.

DOI:10.1016/0378-1097(91)90551-k

PMID:2037229

Abstract

The polymerase chain reaction (PCR) was developed to detect Mycoplasma genitalium. Oligonucleotide primers were used to amplify a 374 bp region of the attachment protein of the mycoplasma. DNA from three strains of M. genitalium tested gave a characteristic PCR product which was not seen with DNA from any other source. As little as 10(-15) g of M. genitalium DNA could be detected and it was found in the vagina of progesterone-treated BALB/c mice inoculated with M. genitalium organisms later than they could be cultured from this site, but not in mice that never became colonised vaginally.

摘要

聚合酶链反应（PCR）被用于检测生殖支原体。使用寡核苷酸引物扩增支原体附着蛋白的一个374bp区域。检测的三株生殖支原体的DNA产生了特征性的PCR产物，而其他来源的DNA则未出现这种情况。能检测到低至10⁻¹⁵g的生殖支原体DNA，并且在接种了生殖支原体的经孕酮处理的BALB/c小鼠阴道中发现了该DNA，其出现时间晚于从该部位培养出该支原体的时间，但未在阴道从未被定殖的小鼠中发现。