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针对影响RNA转录的甲型流感病毒核蛋白的单克隆抗体。

Monoclonal antibodies to the influenza A virus nucleoprotein affecting RNA transcription.

作者信息

van Wyke K L, Bean W J, Webster R G

出版信息

J Virol. 1981 Jul;39(1):313-7. doi: 10.1128/JVI.39.1.313-317.1981.

DOI:10.1128/JVI.39.1.313-317.1981
PMID:6168772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC171292/
Abstract

Monoclonal antibodies were used to map the antigenic domains of the A/WSN/33 (HoN1) influenza virus nucleoprotein. Three nonoverlapping antigenic regions of the nucleoprotein were identified by using competitive-binding enzyme-linked immunosorbent assays. Monoclonal antibodies to two nucleoprotein domains inhibited in vitro transcription of viral RNA, suggesting that these specific regions of the nucleoprotein are topographically or functionally involved in RNA transcription.

摘要

单克隆抗体被用于绘制A/WSN/33（H1N1）流感病毒核蛋白的抗原结构域图谱。通过竞争性结合酶联免疫吸附试验，确定了核蛋白的三个不重叠的抗原区域。针对两个核蛋白结构域的单克隆抗体抑制了病毒RNA的体外转录，这表明核蛋白的这些特定区域在拓扑结构或功能上参与了RNA转录。

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本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Antigenic variation of influenza A virus nucleoprotein detected with monoclonal antibodies.用单克隆抗体检测甲型流感病毒核蛋白的抗原变异
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Transcription of the influenza ribonucleic acid genome by a virion polymerase. 3. Completeness of the transcription process.流感病毒核糖核酸基因组由病毒粒子聚合酶进行转录。3.转录过程的完整性。
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Isolation and preliminary characterization of temperature-sensitive mutants of influenza virus.流感病毒温度敏感突变体的分离与初步鉴定
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Peroxidase-labeled antibody. A new method of conjugation.过氧化物酶标记抗体。一种新的偶联方法。
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