School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden.
J Virol. 2010 Jun;84(12):5868-79. doi: 10.1128/JVI.02383-09. Epub 2010 Apr 7.
Coxsackievirus B2 (CVB2), one of six human pathogens of the group B coxsackieviruses within the enterovirus genus of Picornaviridae, causes a wide spectrum of human diseases ranging from mild upper respiratory illnesses to myocarditis and meningitis. The CVB2 prototype strain Ohio-1 (CVB2O) was originally isolated from a patient with summer grippe in the 1950s. Later on, CVB2O was adapted to cytolytic replication in rhabdomyosarcoma (RD) cells. Here, we present analyses of the correlation between the adaptive mutations of this RD variant and the cytolytic infection in RD cells. Using reverse genetics, we identified a single amino acid change within the exposed region of the VP1 protein (glutamine to lysine at position 164) as the determinant for the acquired cytolytic trait. Moreover, this cytolytic virus induced apoptosis, including caspase activation and DNA degradation, in RD cells. These findings contribute to our understanding of the host cell adaptation process of CVB2O and provide a valuable tool for further studies of virus-host interactions.
柯萨奇病毒 B2（CVB2）是小核糖核酸病毒科肠道病毒属 B 组柯萨奇病毒 6 种人类病原体之一，可引起广泛的人类疾病，从轻度上呼吸道疾病到心肌炎和脑膜炎。CVB2 原型株俄亥俄州 1 号（CVB2O）最初是从 20 世纪 50 年代夏季流感患者中分离出来的。后来，CVB2O 适应了横纹肌肉瘤（RD）细胞的裂解复制。在这里，我们分析了 RD 变异株的适应性突变与 RD 细胞裂解感染之间的相关性。我们使用反向遗传学，在 VP1 蛋白的暴露区域内确定了一个单一的氨基酸变化（164 位的谷氨酰胺突变为赖氨酸）是获得裂解特性的决定因素。此外，这种裂解病毒在 RD 细胞中诱导细胞凋亡，包括半胱天冬酶的激活和 DNA 的降解。这些发现有助于我们理解 CVB2O 的宿主细胞适应过程，并为进一步研究病毒-宿主相互作用提供了有价值的工具。
