School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden.
Virol J. 2011 Oct 14;8:473. doi: 10.1186/1743-422X-8-473.
Colorectal cancer is one of the most common cancers in the world, killing nearly 50% of patients afflicted. Though progress is being made within surgery and other complementary treatments, there is still need for new and more effective treatments. Oncolytic virotherapy, meaning that a cancer is cured by viral infection, is a promising field for finding new and improved treatments. We have investigated the oncolytic potential of several low-pathogenic echoviruses with rare clinical occurrence. Echoviruses are members of the enterovirus genus within the family Picornaviridae.
Six colon cancer cell lines (CaCo-2, HT29, LoVo, SW480, SW620 and T84) were infected by the human enterovirus B species echovirus 12, 15, 17, 26 and 29, and cytopathic effects as well as viral replication efficacy were investigated. Infectivity was also tested in spheroids grown from HT29 cells.
Echovirus 12, 17, 26 and 29 replicated efficiently in almost all cell lines and were considered highly cytolytic. The infectivity of these four viruses was further evaluated in artificial tumors (spheroids), where it was found that echovirus 12, 17 and 26 easily infected the spheroids.
We have found that echovirus 12, 17 and 26 have potential as oncolytic agents against colon cancer, by comparing the cytolytic capacity of five low-pathogenic echoviruses in six colon cancer cell lines and in artificial tumors.
结直肠癌是世界上最常见的癌症之一，近 50%的患者因此死亡。尽管手术和其他辅助治疗方面取得了进展，但仍需要新的、更有效的治疗方法。溶瘤病毒疗法是指通过病毒感染治愈癌症，是寻找新的、改进的治疗方法的一个有前途的领域。我们研究了几种低致病性肠道病毒在罕见临床病例中的溶瘤潜力。肠道病毒是小核糖核酸病毒科肠道病毒属的成员。
用人肠道病毒 B 种柯萨奇病毒 12、15、17、26 和 29 感染六种结肠癌细胞系（CaCo-2、HT29、LoVo、SW480、SW620 和 T84），研究细胞病变效应和病毒复制效果。还在 HT29 细胞生长的球体中测试了感染性。
柯萨奇病毒 12、17、26 和 29 在几乎所有细胞系中都能高效复制，被认为具有高度细胞溶解作用。这四种病毒的感染力在人工肿瘤（球体）中进一步进行了评估，发现柯萨奇病毒 12、17 和 26 很容易感染球体。
通过比较五种低致病性肠道病毒在六种结肠癌细胞系和人工肿瘤中的细胞溶解能力，我们发现柯萨奇病毒 12、17 和 26 具有作为结肠癌溶瘤剂的潜力。
