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脐带基质、脐带血和骨髓间充质干细胞增殖及多系分化潜能的比较。

Comparison of proliferative and multilineage differentiation potentials of cord matrix, cord blood, and bone marrow mesenchymal stem cells.

作者信息

Shetty Prathibha, Cooper Khushnuma, Viswanathan Chandra

机构信息

Regenerative Medicine Group, Reliance Life Sciences Pvt. Ltd., Navi Mumbai, India.

出版信息

Asian J Transfus Sci. 2010 Jan;4(1):14-24. doi: 10.4103/0973-6247.59386.

DOI:10.4103/0973-6247.59386
PMID:20376261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2847339/
Abstract

BACKGROUND

Hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) are the two widely studied and characterized adult stem cells. Thus far, MSCs were obtained from the bone marrow, which is a painful procedure. Therefore, MSCs from less common sources like cord blood, adipose tissue, tooth pulp, and so on, have been the subject of research. The purpose of this study is to explore the possibility of finding MSCs from a less controversial, easy, and abundant source, such as the umbilical cord, for potential regenerative medicine applications.

STUDY DESIGN AND METHODS

Five bone marrow samples (BM), seventy cord blood units (CB), and four umbilical cord matrix (CM) samples have been used for the study. Expanded MSCs were checked for biomarker expression by flow cytometry and were also checked for their differentiation to mesodermal and ectodermal lineages.

RESULTS

MSCs could be isolated from 100% BM and CM samples, as compared to only 6% of CB samples. The fold expansion of the mesenchymal stem cells observed in CB (CB-MSCs) was distinctly higher as compared to BM (BM-MSCs) and CM (CM-MSCs). MSCs isolated from all the three sources expressed a characteristic mesenchymal phenotype of CD45 - /vWF - /CD14 - /CD31 - /CD73 + /CD105 + /SSEA4 + /CD29 + /CD44 + /HLAABC +, whereas, the HLA DR was conspicuously absent in CM-MSCs and CB-MSCs. Although osteogenic, chondrogenic, and neural differentiation was observed in MSCs from all sources, adipogenic differentiation was observed only in BM-MSCs.

CONCLUSION

CM-MSCs are a dependable source of an unlimited number of MSCs for autologous and allogenic use in regenerative medicine.

摘要

背景

造血干细胞（HSCs）和间充质干细胞（MSCs）是两种得到广泛研究和表征的成体干细胞。迄今为止，MSCs是从骨髓中获取的，这是一个痛苦的过程。因此，来自脐带血、脂肪组织、牙髓等不太常见来源的MSCs一直是研究的主题。本研究的目的是探索从脐带这种争议较小、获取容易且来源丰富的来源中找到MSCs用于潜在再生医学应用的可能性。

研究设计与方法

本研究使用了5份骨髓样本（BM）、70份脐带血样本（CB）和4份脐带基质（CM）样本。通过流式细胞术检查扩增后的MSCs的生物标志物表达，并检查它们向中胚层和外胚层谱系的分化情况。

结果

与仅6%的CB样本相比，100%的BM和CM样本中都能分离出MSCs。与BM（BM-MSCs）和CM（CM-MSCs）相比，在CB（CB-MSCs）中观察到的间充质干细胞的扩增倍数明显更高。从所有这三种来源分离出的MSCs都表达CD45 - /vWF - /CD14 - /CD31 - /CD73 + /CD105 + /SSEA4 + /CD29 + /CD44 + /HLAABC +这种特征性的间充质表型，然而，CM-MSCs和CB-MSCs中明显不存在HLA DR。尽管在所有来源的MSCs中都观察到了成骨、软骨生成和神经分化，但仅在BM-MSCs中观察到了脂肪生成分化。

结论

CM-MSCs是再生医学中用于自体和异体使用的无限数量MSCs的可靠来源。

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