Faculty of Science, Department of Chemistry, Trakya University, Section of Biochemistry , Edirne , Turkey.
Artif Cells Nanomed Biotechnol. 2014 Aug;42(4):256-61. doi: 10.3109/21691401.2013.764313. Epub 2013 Jan 30.
In this study, a novel fresh broad (Vicia faba) tissue homogenate-based biosensor for determination of phenolic compounds was developed. The biosensor was constructed by immobilizing tissue homogenate of fresh broad (Vicia faba) on to glassy carbon electrode. For the stability of the biosensor, general immobilization techniques were used to secure the fresh broad tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of fresh broad tissue homogenate and gelatin, glutaraldehyde percentage, optimum pH, optimum temperature and optimum buffer concentration, thermal stability, interference effects, linear range, storage stability, repeatability and sample applications (Wine, beer, fruit juices) were also investigated. Besides, the detection ranges of thirteen phenolic compounds were obtained with the help of the calibration graphs. A typical calibration curve for the sensor revealed a linear range of 5-60 μM catechol. In reproducibility studies, variation coefficient (CV) and standard deviation (SD) were calculated as 1.59%, 0.64×10(-3) μM, respectively.
本研究开发了一种新型的基于新鲜蚕豆组织匀浆的生物传感器，用于测定酚类化合物。该生物传感器通过将新鲜蚕豆组织匀浆固定在玻碳电极上构建而成。为了提高生物传感器的稳定性，采用了常规的固定化技术，将新鲜蚕豆组织固定在明胶-戊二醛交联基质中。在优化和表征研究中，还研究了新鲜蚕豆组织匀浆和明胶的用量、戊二醛的百分比、最佳 pH 值、最佳温度和最佳缓冲液浓度、热稳定性、干扰效应、线性范围、储存稳定性、重复性和样品应用（葡萄酒、啤酒、果汁）。此外，借助校准曲线获得了 13 种酚类化合物的检测范围。传感器的典型校准曲线显示，儿茶酚的线性范围为 5-60 μM。在重现性研究中，变异系数（CV）和标准偏差（SD）分别计算为 1.59%和 0.64×10(-3) μM。
