Department of Forensic Biology, Sichuan University, Chengdu, People's Republic of China.
DNA Cell Biol. 2010 Jul;29(7):381-6. doi: 10.1089/dna.2009.1003.
CD86 (B7-2), one of the costimulatory molecules on antigen-presenting cells, plays essential roles not only in autoimmunity and transplantation but also in tumor immunity. The purpose of this study was to investigate whether CD86 gene polymorphism was involved in predisposing an individual to colorectal cancer (CRC). The CD86 +1057 G/A polymorphism was genotyped by performing polymerase chain reaction-restriction fragment length polymorphism in 273 patients with CRC and 292 healthy controls. There were significant differences in the genotype and allele distribution of +1057 G/A polymorphism of the CD86 gene between cases and controls. The +1057 AA genotype was associated with a significantly increased risk of CRC when compared with the GG genotype (odds ratio [OR] = 2.16; 95% confidence interval [CI], 1.31-3.58). Using the G allele as a reference, a significant correlation was detected between the presence of the A allele and a risk of developing CRC (OR = 1.42; 95% CI, 1.12-1.80). Interestingly, the A allele in female patients with CRC was significantly higher than that in male patients after stratified analysis (OR = 1.49; 95% CI, 1.04-2.14). These data suggest that CD86 +1057G/A polymorphism may contribute to genetic susceptibility to CRC in a Chinese population.
CD86（B7-2）是抗原呈递细胞上的共刺激分子之一，不仅在自身免疫和移植中，而且在肿瘤免疫中都发挥着重要作用。本研究旨在探讨 CD86 基因多态性是否与个体易患结直肠癌（CRC）有关。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测 273 例 CRC 患者和 292 例健康对照者 CD86+1057G/A 多态性。病例组和对照组 CD86 基因+1057G/A 多态性的基因型和等位基因分布存在显著差异。与 GG 基因型相比，+1057AA 基因型与 CRC 的发病风险显著增加（比值比[OR]=2.16；95%置信区间[CI]，1.31-3.58）。以 G 等位基因为参考，发现 A 等位基因与 CRC 发病风险之间存在显著相关性（OR=1.42；95%CI，1.12-1.80）。有趣的是，分层分析显示，CRC 女性患者的 A 等位基因明显高于男性患者（OR=1.49；95%CI，1.04-2.14）。这些数据表明，CD86+1057G/A 多态性可能与中国人群 CRC 的遗传易感性有关。
